The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
19362170
DOI
10.1016/j.intimp.2009.04.005
PII: S1567-5769(09)00136-2
Knihovny.cz E-zdroje
- MeSH
- buněčné extrakty MeSH
- buněčné linie MeSH
- down regulace MeSH
- dusitany metabolismus MeSH
- ethylendiaminy MeSH
- lipidy chemie MeSH
- lipopolysacharidy metabolismus MeSH
- makrofágy účinky léků enzymologie patologie MeSH
- myši MeSH
- nesedativní H1-antihistaminika chemie farmakologie MeSH
- oxid dusnatý metabolismus MeSH
- sulfanilamidy MeSH
- synthasa oxidu dusnatého, typ II metabolismus MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- buněčné extrakty MeSH
- dusitany MeSH
- ethylendiaminy MeSH
- Griess reagent MeSH Prohlížeč
- lipidy MeSH
- lipopolysacharidy MeSH
- nesedativní H1-antihistaminika MeSH
- Nos2 protein, mouse MeSH Prohlížeč
- oxid dusnatý MeSH
- sulfanilamidy MeSH
- synthasa oxidu dusnatého, typ II MeSH
H1-antihistamines are known to be important modulators of inflammatory response. However, the information about the influence of these drugs on reactive nitrogen species generation is still controversial. The main aim of the present study was to investigate the effects of selected H1-antihistamines on nitric oxide production by lipopolysaccharide-stimulated murine macrophages RAW 264.7, measured as changes in inducible nitric oxide synthase (iNOS) protein expression in cell lysates by Western blotting and nitrite formation in cell supernatants using the Griess reaction. In pharmacological non-toxic concentrations, H1-antihistamines significantly inhibited nitrite accumulation that was not caused by the scavenging ability of drugs against nitric oxide, measured amperometrically. The degree of inhibition of nitrite accumulation positively correlated with the degree of tested lipophilicity, measured by reversed-phase thin layer chromatography. Furthermore, H1-antihistamines differentially modulated the iNOS protein expression. In conclusion, as was shown in this study, the modulation of nitric oxide production could be caused by the downregulation of iNOS protein expression and/or the iNOS protein activity.
Citace poskytuje Crossref.org
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