A gene cloning system for the siomycin producer Streptomyces sioyaensis NRRL-B5408
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- antibakteriální látky metabolismus MeSH
- genetické vektory genetika MeSH
- klonování DNA metody MeSH
- konjugace genetická MeSH
- peptidy genetika metabolismus MeSH
- plazmidy genetika MeSH
- reportérové geny MeSH
- Streptomyces genetika metabolismus MeSH
- technika přenosu genů * MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- peptidy MeSH
- siomycin MeSH Prohlížeč
Streptomyces sioyaensis NRRL-B5408 produces a siomycin complex (a group of thiopeptide antibiotics structurally related to thiostrepton). Development of genetic tools for the detection of siomycin production and DNA transfer into this strain is described. The existing tipA-based reporter system for determination of siomycin production was modified to achieve its stable integration into actinomycete genomes. Various replicative plasmids (pKC1139, pKC1218E, pSOK101) as well as actinophage phi C31- and VWB-based vectors pSET152 and pSOK804, respectively, were conjugally transferred from E. coli into the siomycin producer at a frequency ranging from 3.7 x 10(-9) to 1.1 x 10(-5). The transconjugants did not differ from wild type in their ability to produce siomycin. There is one attB site for each integrative plasmid. The utility of temperature sensitive replicon of pKC1139 for insertional gene inactivation in S. sioyaensis has been validated by disruption of putative nonribosomal peptide synthetase gene.
Zobrazit více v PubMed
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