A gene cloning system for the siomycin producer Streptomyces sioyaensis NRRL-B5408

. 2009 ; 54 (2) : 91-6. [epub] 20090506

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid19418244

Streptomyces sioyaensis NRRL-B5408 produces a siomycin complex (a group of thiopeptide antibiotics structurally related to thiostrepton). Development of genetic tools for the detection of siomycin production and DNA transfer into this strain is described. The existing tipA-based reporter system for determination of siomycin production was modified to achieve its stable integration into actinomycete genomes. Various replicative plasmids (pKC1139, pKC1218E, pSOK101) as well as actinophage phi C31- and VWB-based vectors pSET152 and pSOK804, respectively, were conjugally transferred from E. coli into the siomycin producer at a frequency ranging from 3.7 x 10(-9) to 1.1 x 10(-5). The transconjugants did not differ from wild type in their ability to produce siomycin. There is one attB site for each integrative plasmid. The utility of temperature sensitive replicon of pKC1139 for insertional gene inactivation in S. sioyaensis has been validated by disruption of putative nonribosomal peptide synthetase gene.

Zobrazit více v PubMed

J Am Chem Soc. 2005 Nov 2;127(43):15042-4 PubMed

Mol Microbiol. 2005 Dec;58(5):1276-87 PubMed

Chem Rev. 2005 Feb;105(2):685-714 PubMed

J Antibiot (Tokyo). 2004 Sep;57(9):590-6 PubMed

Cancer Res. 2006 Oct 1;66(19):9731-5 PubMed

Microb Ecol. 2005 Jan;49(1):10-24 PubMed

Microbiology (Reading). 2000 Mar;146 ( Pt 3):611-619 PubMed

Mol Biochem Parasitol. 2000 Jun;109(1):17-23 PubMed

FEMS Microbiol Lett. 2003 Aug 8;225(1):53-7 PubMed

J Bacteriol. 2004 Mar;186(5):1345-54 PubMed

EMBO J. 1993 Aug;12(8):3183-91 PubMed

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...