Different inhibition of acetylcholinesterase in selected parts of the rat brain following intoxication with VX and Russian VX
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- acetylcholinesterasa účinky léků metabolismus MeSH
- chemické bojové látky otrava MeSH
- cholinesterasové inhibitory otrava MeSH
- injekce intramuskulární MeSH
- krysa rodu Rattus MeSH
- LD50 MeSH
- mozek účinky léků enzymologie MeSH
- organothiofosforové sloučeniny otrava MeSH
- potkani Wistar MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- acetylcholinesterasa MeSH
- chemické bojové látky MeSH
- cholinesterasové inhibitory MeSH
- organothiofosforové sloučeniny MeSH
- S-(N,N-diethylaminoethyl) isobutyl methylphosphothiolate MeSH Prohlížeč
- VX MeSH Prohlížeč
Differences between acetylcholinesterase (AChE) inhibition in the brain structures following VX and RVX exposure are not known as well as information on the possible correlation of biochemical and histochemical methods detecting AChE activity. Therefore, inhibition of AChE in different brain parts detected by histochemical and biochemical techniques was compared in rats intoxicated with VX and RVX. AChE activities in defined brain regions 30 min after treating rats with VX and Russian VX intramuscularly (1.0 x LD(50)) were determined by using biochemical and histochemical methods. AChE inhibition was less expressed for RVX, in comparison with VX. Frontal cortex and pontomedullar areas containing ncl. reticularis has been found as the most sensitive areas for the action of VX. For RVX, these structures were determined to be frontal cortex, dorsal septum, and hippocampus, respectively. Histochemical and biochemical results were in good correlation (R(xy) = 0.8337). Determination of AChE activity in defined brain structures was a more sensitive parameter for VX or RVX exposure than the determination of AChE activity in the whole-brain homogenate. This activity represents a "mean" of the activities in different structures. Thus, AChE activity is the main parameter investigated in studies searching for target sites following nerve-agent poisoning contributing to better understanding of toxicodynamics of nerve agents.
Citace poskytuje Crossref.org
