Increased production of proinflammatory cytokines in adipose tissue of patients with end-stage renal disease
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19539174
DOI
10.1016/j.nut.2008.12.012
PII: S0899-9007(09)00115-4
Knihovny.cz E-resources
- MeSH
- Adiponectin genetics metabolism MeSH
- Antigens, Differentiation, Myelomonocytic metabolism MeSH
- C-Reactive Protein metabolism MeSH
- Antigens, CD metabolism MeSH
- Chemokine CCL2 genetics metabolism MeSH
- Kidney Failure, Chronic metabolism MeSH
- Cytokines metabolism MeSH
- Interleukin-6 genetics metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- Inflammation Mediators metabolism MeSH
- RNA, Messenger biosynthesis MeSH
- Intra-Abdominal Fat metabolism MeSH
- Subcutaneous Fat metabolism MeSH
- Receptors, Adiponectin biosynthesis genetics MeSH
- Resistin genetics metabolism MeSH
- Tumor Necrosis Factor-alpha blood MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adiponectin MeSH
- ADIPOR1 protein, human MeSH Browser
- Antigens, Differentiation, Myelomonocytic MeSH
- C-Reactive Protein MeSH
- Antigens, CD MeSH
- CD68 antigen, human MeSH Browser
- Chemokine CCL2 MeSH
- Cytokines MeSH
- Interleukin-6 MeSH
- Inflammation Mediators MeSH
- RNA, Messenger MeSH
- Receptors, Adiponectin MeSH
- Resistin MeSH
- Tumor Necrosis Factor-alpha MeSH
OBJECTIVE: The number of patients with end-stage renal disease (ESRD) is rising and these patients are at higher risk of cardiovascular disease. We studied the role of hormonal production of adipose tissue in the development of chronic inflammation in patients with ESRD before kidney transplantation. METHODS: Fifteen women with ESRD and 17 healthy women (control) underwent single blood drawing and visceral and subcutaneous adipose tissue sampling during surgery (kidney transplantation in the ESRD group or cholecystectomy in the control group). Serum concentrations of C-reactive protein, interleukin-6, tumor necrosis factor-alpha, leptin, adiponectin, resistin, monocyte chemoattractant protein-1 were measured. Messenger RNA expression of the same hormones, adiponectin receptors 1 and 2 and immunocompetent cell marker CD68 in subcutaneous and visceral samples were measured using real-time polymerase chain reaction. Adipose tissue was examined immunohistochemically for CD68-positive cells. RESULTS: Serum concentrations of C-reactive protein, adiponectin, resistin, interleukin-6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 were significantly higher in the ESRD versus control group. Subcutaneous and visceral mRNA expressions of tumor necrosis factor-alpha and CD68 were significantly increased in the ESRD versus control group. Adiponectin receptor-1 and monocyte chemoattractant protein-1 mRNA expressions were significantly higher in visceral but not in subcutaneous adipose tissue of the ESRD group. Messenger RNA expressions of resistin, leptin, adiponectin, interleukin-6, and adiponectin receptor-2 in both fat depots did not significantly differ between groups. Increased infiltration of subcutaneous and visceral adipose tissue with CD68-positive immunocompetent cells was found in the ESRD group by histologic examination. CONCLUSION: Subcutaneous and visceral adipose tissues in ESRD express higher amounts of proinflammatory cytokines and may play a role in the development of systemic inflammation.
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