Increased production of proinflammatory cytokines in adipose tissue of patients with end-stage renal disease
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
19539174
DOI
10.1016/j.nut.2008.12.012
PII: S0899-9007(09)00115-4
Knihovny.cz E-zdroje
- MeSH
- adiponektin genetika metabolismus MeSH
- antigeny diferenciační myelomonocytární metabolismus MeSH
- C-reaktivní protein metabolismus MeSH
- CD antigeny metabolismus MeSH
- chemokin CCL2 genetika metabolismus MeSH
- chronické selhání ledvin metabolismus MeSH
- cytokiny metabolismus MeSH
- interleukin-6 genetika metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- mediátory zánětu metabolismus MeSH
- messenger RNA biosyntéza MeSH
- nitrobřišní tuk metabolismus MeSH
- podkožní tuk metabolismus MeSH
- receptory adiponektinu biosyntéza genetika MeSH
- resistin genetika metabolismus MeSH
- TNF-alfa krev MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adiponektin MeSH
- ADIPOR1 protein, human MeSH Prohlížeč
- antigeny diferenciační myelomonocytární MeSH
- C-reaktivní protein MeSH
- CD antigeny MeSH
- CD68 antigen, human MeSH Prohlížeč
- chemokin CCL2 MeSH
- cytokiny MeSH
- interleukin-6 MeSH
- mediátory zánětu MeSH
- messenger RNA MeSH
- receptory adiponektinu MeSH
- resistin MeSH
- TNF-alfa MeSH
OBJECTIVE: The number of patients with end-stage renal disease (ESRD) is rising and these patients are at higher risk of cardiovascular disease. We studied the role of hormonal production of adipose tissue in the development of chronic inflammation in patients with ESRD before kidney transplantation. METHODS: Fifteen women with ESRD and 17 healthy women (control) underwent single blood drawing and visceral and subcutaneous adipose tissue sampling during surgery (kidney transplantation in the ESRD group or cholecystectomy in the control group). Serum concentrations of C-reactive protein, interleukin-6, tumor necrosis factor-alpha, leptin, adiponectin, resistin, monocyte chemoattractant protein-1 were measured. Messenger RNA expression of the same hormones, adiponectin receptors 1 and 2 and immunocompetent cell marker CD68 in subcutaneous and visceral samples were measured using real-time polymerase chain reaction. Adipose tissue was examined immunohistochemically for CD68-positive cells. RESULTS: Serum concentrations of C-reactive protein, adiponectin, resistin, interleukin-6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 were significantly higher in the ESRD versus control group. Subcutaneous and visceral mRNA expressions of tumor necrosis factor-alpha and CD68 were significantly increased in the ESRD versus control group. Adiponectin receptor-1 and monocyte chemoattractant protein-1 mRNA expressions were significantly higher in visceral but not in subcutaneous adipose tissue of the ESRD group. Messenger RNA expressions of resistin, leptin, adiponectin, interleukin-6, and adiponectin receptor-2 in both fat depots did not significantly differ between groups. Increased infiltration of subcutaneous and visceral adipose tissue with CD68-positive immunocompetent cells was found in the ESRD group by histologic examination. CONCLUSION: Subcutaneous and visceral adipose tissues in ESRD express higher amounts of proinflammatory cytokines and may play a role in the development of systemic inflammation.
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