Metoda merení parametrů metabolismu proteinů na izolovaných kosterních svalech laboratorního potkana
[Method of measurement of protein metabolism in isolated skeletal muscle of the rat]
Jazyk čeština Země Česko Médium print
Typ dokumentu anglický abstrakt, časopisecké články
PubMed
19569589
- MeSH
- kosterní svaly metabolismus MeSH
- krysa rodu Rattus MeSH
- leucin metabolismus MeSH
- oxidace-redukce MeSH
- potkani Wistar MeSH
- svalové proteiny metabolismus MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- leucin MeSH
- svalové proteiny MeSH
The aim of our present research is to evaluate the direct effects of humoral factors on protein metabolism. For this purpose we have adopted a method that enables measuring of parameters of protein and amino acid metabolism in isolated m. soleus and m. extensor digitorum longus of the rat. The rate of tyrosine release was used to estimate protein breakdown. The oxidation of leucine was evaluated by the rate of 14CO2 production from [1-14C]leucine. Incorporation of [1-14C]leucine into muscle proteins was used to estimate protein synthesis. The metabolic state of the muscles was evaluated by measuring changes in ATP, ADP, AMP levels, and calculated energy charge.
