Metoda merení parametrů metabolismu proteinů na izolovaných kosterních svalech laboratorního potkana
[Method of measurement of protein metabolism in isolated skeletal muscle of the rat]
Language Czech Country Czech Republic Media print
Document type English Abstract, Journal Article
PubMed
19569589
- MeSH
- Muscle, Skeletal metabolism MeSH
- Rats MeSH
- Leucine metabolism MeSH
- Oxidation-Reduction MeSH
- Rats, Wistar MeSH
- Muscle Proteins metabolism MeSH
- In Vitro Techniques MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Names of Substances
- Leucine MeSH
- Muscle Proteins MeSH
The aim of our present research is to evaluate the direct effects of humoral factors on protein metabolism. For this purpose we have adopted a method that enables measuring of parameters of protein and amino acid metabolism in isolated m. soleus and m. extensor digitorum longus of the rat. The rate of tyrosine release was used to estimate protein breakdown. The oxidation of leucine was evaluated by the rate of 14CO2 production from [1-14C]leucine. Incorporation of [1-14C]leucine into muscle proteins was used to estimate protein synthesis. The metabolic state of the muscles was evaluated by measuring changes in ATP, ADP, AMP levels, and calculated energy charge.
