Identification of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in patients with erythema migrans
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anaplasma phagocytophilum immunology isolation & purification MeSH
- Antigens, Surface genetics MeSH
- Bacterial Vaccines genetics MeSH
- Borrelia burgdorferi genetics immunology isolation & purification MeSH
- Diagnosis, Differential MeSH
- Adult MeSH
- Ehrlichiosis blood diagnosis microbiology MeSH
- Erythema Chronicum Migrans blood diagnosis microbiology MeSH
- HL-60 Cells MeSH
- Pregnancy Complications, Infectious diagnosis microbiology MeSH
- Middle Aged MeSH
- Humans MeSH
- Lipoproteins genetics MeSH
- Lyme Disease blood diagnosis microbiology MeSH
- Adolescent MeSH
- Placenta microbiology MeSH
- Bacterial Outer Membrane Proteins genetics MeSH
- Antibodies, Bacterial blood MeSH
- Aged MeSH
- Pregnancy MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Aged MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Surface MeSH
- Bacterial Vaccines MeSH
- Lipoproteins MeSH
- OspA protein MeSH Browser
- Bacterial Outer Membrane Proteins MeSH
- Antibodies, Bacterial MeSH
Anaplasma phagocytophilum has been first isolated from the blood of two Czech patients simultaneously with a cultivation of Borrelia burgdorferi sensu lato from their erythema migrans lesions. Cultivation of different Borrelia spp. from 12 erythema migrans biopsies, from 2 blood, one liquor and one placenta sample in BSK-H medium was successful. Adapted conventional methods targeting 16S rRNA and OspA genes for real-time polymerase chain reaction (PCR) and partial sequencing of these genes together with microscopical examinations of the blood smears provided a direct detection of the B. afzelii, B. burgdorferi, B. garinii, B. valaisiana and B. bissettii in the skin, B. garinii in the blood, placenta and liquor in 24 (36.3 %) patients, and A. phagocytophilum in 10 (15 %) patients with erythema migrans. Positive indirect IgM immunofluorescence against Anaplasma sp. was obtained in 7 cases, specific IgG antibodies were detected in 12 patients. Three women suffering from erythema migrans in the first trimester had positive PCR for Anaplasma and/or for Borrelia in the blood and two of them, later, in the placenta. Interpretation of laboratory data can bring important contribution to establishing the role of Anaplasma sp. in erythema migrans and forming the principle of precaution with laboratory diagnosis during pregnancy which always should be reflected in the resistance of Anaplasma sp. toward penicillins.
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