Molecular weight and polydispersity of calf-thymus DNA: static light-scattering and size-exclusion chromatography with dual detection
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
19817429
DOI
10.1021/bm900768j
Knihovny.cz E-zdroje
- MeSH
- DNA analýza chemie MeSH
- gelová chromatografie * metody MeSH
- molekulová hmotnost MeSH
- radiační rozptyl MeSH
- skot MeSH
- světlo * MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- calf thymus DNA MeSH Prohlížeč
- DNA MeSH
Plausible calf-thymus DNA molecular weight distribution can be obtained by size-exclusion chromatography with dual low-angle light scattering/refractometric detection at sufficiently low flow rate. The distribution extends over three decades of molecular weight and is characterized by weight average molecular weight M(w) = 8418000 and polydispersity index M(w)/M(n) = 5.2. After strongly scattering impurities had been removed from the sample using adsorption properties of the 3 mum mixed-cellulose-ester filter membranes, static light-scattering measurement in flow injection mode was feasible and gave M(w) = 8580000, corroborating the veracity of SEC results.
Citace poskytuje Crossref.org
