Immunoregulatory properties of mouse limbal stem cells
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
20065115
DOI
10.4049/jimmunol.0903049
PII: jimmunol.0903049
Knihovny.cz E-resources
- MeSH
- Cytokines antagonists & inhibitors biosynthesis MeSH
- Cytotoxicity Tests, Immunologic methods MeSH
- Immunologic Factors biosynthesis isolation & purification physiology MeSH
- Immunosuppressive Agents isolation & purification metabolism pharmacology MeSH
- Growth Inhibitors biosynthesis isolation & purification physiology MeSH
- Stem Cells chemistry cytology immunology MeSH
- Cells, Cultured MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Cell Proliferation MeSH
- Epithelium, Corneal chemistry cytology immunology MeSH
- Cell Separation methods MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Cytokines MeSH
- Immunologic Factors MeSH
- Immunosuppressive Agents MeSH
- Growth Inhibitors MeSH
Stem cells have been demonstrated in nearly all adult mammalian tissues and play a vital role in their physiological renewal and healing after injury. Due to their irreplaceable role in tissue repair, these cells had to develop mechanisms protecting them from deleterious inflammatory immune reactions and ensuring their increased resistance to various apoptosis-inducing agents. In this study, we demonstrate that a population of mouse limbal cells highly enriched for cells expressing markers and characteristics of limbal stem cells (LSCs) suppresses in a dose-dependent manner the proliferation of lymphocytes elicited by mitogens or TCR-triggering and significantly inhibits the production of proinflammatory cytokines by activated T cells. The suppression was mediated by soluble factor(s) and did not affect early cell activation. LSCs were even more suppressive than mesenchymal stem cells or natural regulatory T cells. In addition, the cells expressing markers and characteristics of LSC had significantly higher levels of mRNA for Fas ligand and for the antiapoptotic molecules Mcl-1, XIAP, and survivin than other limbal cell populations. LSCs were also more resistant to staurosporin-induced apoptotic cell death and to cell-mediated cytotoxic reaction than other limbal cells. Collectively, these results suggest that SC isolated from fresh adult limbal tissue possess immunomodulatory properties and inhibit proinflammatory immune reactions. Simultaneously, these cells express high levels of mRNA for antiapoptotic molecules, which can protect them against cell-mediated cytotoxic reactions and various apoptosis-inducing agents.
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