Immunoregulatory properties of mouse limbal stem cells
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
20065115
DOI
10.4049/jimmunol.0903049
PII: jimmunol.0903049
Knihovny.cz E-zdroje
- MeSH
- cytokiny antagonisté a inhibitory biosyntéza MeSH
- cytotoxické testy imunologické metody MeSH
- imunologické faktory biosyntéza izolace a purifikace fyziologie MeSH
- imunosupresiva izolace a purifikace metabolismus farmakologie MeSH
- inhibitory růstu biosyntéza izolace a purifikace fyziologie MeSH
- kmenové buňky chemie cytologie imunologie MeSH
- kultivované buňky MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- proliferace buněk MeSH
- rohovkový epitel chemie cytologie imunologie MeSH
- separace buněk metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- cytokiny MeSH
- imunologické faktory MeSH
- imunosupresiva MeSH
- inhibitory růstu MeSH
Stem cells have been demonstrated in nearly all adult mammalian tissues and play a vital role in their physiological renewal and healing after injury. Due to their irreplaceable role in tissue repair, these cells had to develop mechanisms protecting them from deleterious inflammatory immune reactions and ensuring their increased resistance to various apoptosis-inducing agents. In this study, we demonstrate that a population of mouse limbal cells highly enriched for cells expressing markers and characteristics of limbal stem cells (LSCs) suppresses in a dose-dependent manner the proliferation of lymphocytes elicited by mitogens or TCR-triggering and significantly inhibits the production of proinflammatory cytokines by activated T cells. The suppression was mediated by soluble factor(s) and did not affect early cell activation. LSCs were even more suppressive than mesenchymal stem cells or natural regulatory T cells. In addition, the cells expressing markers and characteristics of LSC had significantly higher levels of mRNA for Fas ligand and for the antiapoptotic molecules Mcl-1, XIAP, and survivin than other limbal cell populations. LSCs were also more resistant to staurosporin-induced apoptotic cell death and to cell-mediated cytotoxic reaction than other limbal cells. Collectively, these results suggest that SC isolated from fresh adult limbal tissue possess immunomodulatory properties and inhibit proinflammatory immune reactions. Simultaneously, these cells express high levels of mRNA for antiapoptotic molecules, which can protect them against cell-mediated cytotoxic reactions and various apoptosis-inducing agents.
Citace poskytuje Crossref.org
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