Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20138138
DOI
10.1016/j.tiv.2010.01.012
PII: S0887-2333(10)00014-7
Knihovny.cz E-resources
- MeSH
- Annexin A5 MeSH
- Apoptosis drug effects MeSH
- Coloring Agents MeSH
- Benzophenanthridines pharmacology MeSH
- Cell Cycle drug effects MeSH
- DNA Fragmentation drug effects MeSH
- In Situ Nick-End Labeling MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Oxidative Stress drug effects MeSH
- Oxidants toxicity MeSH
- Hydrogen Peroxide antagonists & inhibitors toxicity MeSH
- Cell Proliferation drug effects MeSH
- Propidium MeSH
- Antineoplastic Agents * MeSH
- Reactive Oxygen Species metabolism MeSH
- Tetrazolium Salts MeSH
- Thiazoles MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Annexin A5 MeSH
- Coloring Agents MeSH
- Benzophenanthridines MeSH
- chelilutine MeSH Browser
- Oxidants MeSH
- Hydrogen Peroxide MeSH
- Propidium MeSH
- Antineoplastic Agents * MeSH
- Reactive Oxygen Species MeSH
- sanguilutine MeSH Browser
- Tetrazolium Salts MeSH
- Thiazoles MeSH
- thiazolyl blue MeSH Browser
Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their antiproliferative activities with regard to their ability to induce oxidative stress. We observed potent antiproliferative activities for both alkaloids against three tumour (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and unequal sensitivity with regard to individual cell lines, although independent of the character of the cell line (i.e. tumour vs. normal). Dihydrofluorescein diacetate staining was used to measure intracellular ROS accumulation after treatment with sanguilutine, chelilutine, sanguinarine, and chelerythrine. In addition, anti-oxidative effects were studied. The effects of the alkaloids were compared with the effects of commonly used anti-oxidants, such as trolox, caffeine acid, and chlorogenic acid. None of the tested alkaloids (0.1 and 1 microg/ml) increased ROS production. Pre-incubation of sanguinarine and chelilutine (at all tested concentrations) and sanguilutine and chelerythrine (1 microg/ml) decreased oxidative stress caused by H(2)O(2). These findings indicate high antiproliferative and pro-apoptotic effects of sanguilutine and chelilutine that are not accompanied by oxidative stress induction, to the contrary, both alkaloids showed anti-oxidative effects.
References provided by Crossref.org
Quaternary Benzophenanthridine Alkaloids Act as Smac Mimetics and Overcome Resistance to Apoptosis
Seasonal variation of bioactive alkaloid contents in Macleaya microcarpa (Maxim.) Fedde
