Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20165813
DOI
10.1039/b924072a
Knihovny.cz E-resources
- MeSH
- Nucleic Acid Denaturation MeSH
- DNA-Directed DNA Polymerase metabolism MeSH
- DNA chemistry metabolism MeSH
- Nucleosides chemical synthesis chemistry MeSH
- Nucleotides chemical synthesis chemistry metabolism MeSH
- Thermococcaceae enzymology MeSH
- Bile Acids and Salts chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA-Directed DNA Polymerase MeSH
- DNA MeSH
- Nucleosides MeSH
- Nucleotides MeSH
- Bile Acids and Salts MeSH
Aqueous Sonogashira cross-coupling reactions of 5-iodopyrimidine or 7-iodo-7-deazaadenine nucleosides with bile acid-derived terminal acetylenes linked via an ester or amide tether gave the corresponding bile acid-nucleoside conjugates. Analogous reactions of halogenated nucleoside triphosphates gave directly bile acid-modified dNTPs. Enzymatic incorporation of these modified nucleotides to DNA was successfully performed using Phusion polymerase for primer extension. One of the dNTPs (dCTP bearing cholic acid) was also efficient for PCR amplification.
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