Expression of heat shock protein 70 and NKG2D ligands in acute myeloid leukemia cell lines
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- akutní myeloidní leukemie metabolismus MeSH
- buněčná membrána metabolismus MeSH
- buňky NK cytologie MeSH
- dospělí MeSH
- HL-60 buňky MeSH
- kohortové studie MeSH
- lektinové receptory NK-buněk - podrodina K metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- ligandy MeSH
- mladiství MeSH
- nádorové buněčné linie MeSH
- proteiny tepelného šoku HSP70 metabolismus MeSH
- průtoková cytometrie metody MeSH
- regulace genové exprese u leukemie * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- KLRK1 protein, human MeSH Prohlížeč
- lektinové receptory NK-buněk - podrodina K MeSH
- ligandy MeSH
- proteiny tepelného šoku HSP70 MeSH
UNLABELLED: Membrane-bound heat shock protein 70 (HSP70) was found to be tumor-specific and was proposed as a target for immunotherapy. In the present study, we analyzed cell surface and relative gene expression of HSP70 in cell lines established from patients with different acute myeloid leukemia (AML) subtypes, together with the expression of natural killer (NK) cell activation/inhibitory ligands. MATERIALS AND METHODS: Six AML cell lines were included in this study. The relative gene expression of HSP70 was analyzed using the real-time reverse-transcriptase polymerase chain reaction. Surface expression of HSP70 and NK cell ligands was analyzed using flow cytometry. RESULTS: All cell lines overexpressed HSP70; however, its mRNA levels were not elevated. The expression of NKG2D activation ligands was heterogeneous. CONCLUSION: Our study is the first to describe long-term stationary cell surface expression of HSP70 in different subtypes of AML. Combined with the results of the gene expression experiments these data provide more evidence to the idea of a self-limiting mechanism for HSP70 expression.
Citace poskytuje Crossref.org
Heat-shock protein expression in leukemia
