Flubendazole metabolism and biotransformation enzymes activities in healthy sheep and sheep with haemonchosis
Language English Country Great Britain, England Media print
Document type Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
PubMed
20444026
DOI
10.1111/j.1365-2885.2009.01112.x
PII: JVP1112
Knihovny.cz E-resources
- MeSH
- Biotransformation MeSH
- Haemonchus drug effects MeSH
- Haemonchiasis drug therapy metabolism veterinary MeSH
- Liver enzymology metabolism MeSH
- Mebendazole analogs & derivatives metabolism therapeutic use MeSH
- Sheep Diseases metabolism MeSH
- Sheep MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Controlled Clinical Trial MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- flubendazole MeSH Browser
- Mebendazole MeSH
The aim of this project was to study the influence of haemonchosis, a common parasitic infection of small ruminants caused by Haemonchus contortus, on the activity of biotransformation enzymes and on in vitro flubendazole (FLU) biotransformation in liver and small intestine of lambs (Ovis aries). Twelve lambs were divided into three groups: non-infected animals, animals orally infected with larvae of H. contortus ISE strain for 7 weeks and for 11 weeks. At the end of the experiment, hepatic and intestinal subcellular fractions were prepared and used for assays of biotransformation enzymes activities and FLU metabolism testing. The activities of hepatic cytochromes P450, flavine monooxygenases and carbonyl-reducing enzymes were decreased in infected animals. UDP-glucuronosyl transferase activity was significantly lower (by 35%) in 11 weeks infected animals than that in control animals. When in vitro metabolism of FLU was compared in control and infected animals, significantly lower velocity of FLU reduction was found in infected animals. Slower FLU reduction may be beneficial for the haemonchosis treatment using FLU, because FLU will remain longer in the organism and could cause longer contact of parasites with FLU.
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