Stimulated by demands of the natural environment conservation, the need for thorough structural and functional identification of microorganisms colonizing different ecosystems has contributed to an intensive advance in research techniques. The article shows that some of these techniques are also a convenient tool for determination of the physiological state of single cells in a community of microorganisms. The paper presents selected fluorescent techniques, which are used in research on soil, water and sediment microorganisms. It covers the usability of determination of the dehydrogenase activity of an individual bacterial cell (CTC+) and of bacteria with intact, functioning cytoplasmic membranes, bacteria with an integrated nucleiod (NuCC+) as well as fluorescent in situ hybridization (FISH).

Department of Microbiology Faculty of Biology University of Warmia and Mazury 10 717 Olsztyn Poland

Zobrazit více v PubMed

Appl Environ Microbiol. 2003 Jan;69(1):641-3 PubMed

Int J Food Microbiol. 2000 Apr 10;55(1-3):193-200 PubMed

Appl Environ Microbiol. 1996 Dec;62(12):4587-93 PubMed

FEMS Microbiol Lett. 2005 May 15;246(2):151-8 PubMed

Appl Environ Microbiol. 1999 Apr;65(4):1753-61 PubMed

J Microbiol Methods. 1999 Sep;37(3):231-43 PubMed

Microbiol Rev. 1995 Mar;59(1):143-69 PubMed

Appl Environ Microbiol. 1999 Jun;65(6):2409-17 PubMed

Biotechnol Lett. 2004 Mar;26(5):379-83 PubMed

Nucleic Acids Res. 2007 Jan;35(Database issue):D800-4 PubMed

Folia Microbiol (Praha). 2003;48(2):253-6 PubMed

Microb Ecol. 1977 Jun;4(2):127-33 PubMed

Appl Environ Microbiol. 1994 Aug;60(8):2869-75 PubMed

Appl Environ Microbiol. 2001 Feb;67(2):961-8 PubMed

Pest Manag Sci. 2005 Sep;61(9):863-9 PubMed

Appl Environ Microbiol. 1997 Aug;63(8):3268-73 PubMed

J Microbiol. 2004 Dec;42(4):285-91 PubMed

Science. 1989 Mar 10;243(4896):1360-3 PubMed

Appl Environ Microbiol. 1990 Jun;56(6):1919-25 PubMed

FEMS Microbiol Lett. 2005 Dec 1;253(1):55-8 PubMed

J Appl Bacteriol. 1996 Feb;80(2):209-15 PubMed

Appl Environ Microbiol. 2004 Apr;70(4):2072-8 PubMed

Microbiology (Reading). 1998 Sep;144 ( Pt 9):2427-2439 PubMed

Microbes Infect. 2000 Oct;2(12):1523-35 PubMed

J Microbiol Methods. 2007 Jun;69(3):451-60 PubMed

Microbiology (Reading). 1994 Oct;140 ( Pt 10):2849-58 PubMed

Appl Environ Microbiol. 2004 Jan;70(1):550-7 PubMed

Syst Appl Microbiol. 1999 Sep;22(3):434-44 PubMed

J Microbiol Methods. 2000 Jul;41(2):85-112 PubMed

Nat Rev Microbiol. 2008 May;6(5):339-48 PubMed

Microbiol Mol Biol Rev. 2005 Sep;69(3):440-61 PubMed

Appl Environ Microbiol. 1995 Jun;61(6):2180-5 PubMed

Cytometry. 1993;14(2):136-43 PubMed

Syst Appl Microbiol. 1999 May;22(2):186-96 PubMed

Appl Environ Microbiol. 2004 Nov;70(11):6884-6 PubMed

Folia Microbiol (Praha). 2004;49(4):423-9 PubMed

Appl Environ Microbiol. 2000 Jun;66(6):2589-98 PubMed

Appl Environ Microbiol. 1999 Oct;65(10):4475-83 PubMed

Appl Microbiol Biotechnol. 2006 Dec;73(3):485-94 PubMed

Appl Environ Microbiol. 2008 Mar;74(6):1767-79 PubMed

FEMS Microbiol Ecol. 2003 May 1;44(1):3-15 PubMed

Appl Environ Microbiol. 2006 Mar;72(3):1719-28 PubMed

Appl Environ Microbiol. 1998 Dec;64(12):4973-82 PubMed

Microbiology (Reading). 1998 Oct;144 ( Pt 10):2783-2790 PubMed

Microb Ecol. 1997 Sep;34(2):144-54 PubMed

J Microbiol Methods. 2001 Jan;43(3):213-22 PubMed

FEMS Microbiol Ecol. 2001 Apr;35(2):171-179 PubMed

FEMS Microbiol Ecol. 2006 Feb;55(2):287-98 PubMed

Appl Environ Microbiol. 1999 Aug;65(8):3721-6 PubMed

Mol Microbiol. 2004 Jan;51(1):89-96 PubMed

Biotechnol J. 2008 Jul;3(7):890-903 PubMed

Appl Environ Microbiol. 1978 Dec;36(6):926-35 PubMed

Lett Appl Microbiol. 2006 Apr;42(4):338-43 PubMed

Appl Environ Microbiol. 2003 Mar;69(3):1748-58 PubMed

Microb Ecol. 2006 Jan;51(1):117-27 PubMed

J Microbiol Methods. 2001 Mar 1;44(2):97-103 PubMed

Appl Environ Microbiol. 2002 Jul;68(7):3509-13 PubMed

J Microbiol Methods. 2001 Dec;47(3):281-92 PubMed

Folia Microbiol (Praha). 2005;50(2):161-6 PubMed

Folia Microbiol (Praha). 2009;54(4):263-72 PubMed

Mol Cell Probes. 2006 Apr;20(2):141-6 PubMed

Appl Environ Microbiol. 2007 May;73(9):2956-62 PubMed

Appl Environ Microbiol. 2009 May;75(10):3352-4 PubMed

Appl Environ Microbiol. 1992 Jun;58(6):1801-8 PubMed

Aquat Toxicol. 2008 Feb 18;86(3):352-60 PubMed

Curr Opin Microbiol. 2003 Jun;6(3):302-9 PubMed

Syst Appl Microbiol. 1998 Dec;21(4):579-87 PubMed

Biodegradation. 2002;13(4):285-95 PubMed

J Microbiol Methods. 2003 Jan;52(1):59-68 PubMed

J Microbiol Methods. 1999 Jul;37(1):77-86 PubMed

Symp Ser Soc Appl Microbiol. 1999;85(28):193S-200S PubMed

Appl Environ Microbiol. 1995 Sep;61(9):3367-72 PubMed

Microb Ecol. 2002 Mar;43(2):232-41 PubMed

Appl Environ Microbiol. 2003 Aug;69(8):4935-41 PubMed

J Microbiol Methods. 2003 Dec;55(3):541-55 PubMed

Microb Ecol. 2008 Feb;55(2):270-9 PubMed

FEMS Microbiol Ecol. 2003 Oct 1;46(1):31-8 PubMed

J Microbiol Methods. 2003 Aug;54(2):257-67 PubMed

Appl Environ Microbiol. 2002 Jun;68(6):3094-101 PubMed

J Appl Microbiol. 2004;96(4):684-92 PubMed

Appl Environ Microbiol. 1995 Jul;61(7):2696-701 PubMed

Folia Microbiol (Praha). 2005;50(5):437-42 PubMed

Seasonal changes of microbial communities in two shallow peat bog lakes

Principal methods for isolation and identification of soil microbial communities