Effect of cytoskeleton inhibitors on conidiogenesis and capsule in the long neck yeast Fellomyces examined by scanning electron microscopy
Jazyk angličtina Země Švýcarsko Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
20551635
DOI
10.1159/000316330
PII: 000316330
Knihovny.cz E-zdroje
- MeSH
- Basidiomycota účinky léků ultrastruktura MeSH
- bicyklické sloučeniny heterocyklické farmakologie MeSH
- cytoskelet účinky léků ultrastruktura MeSH
- mikroskopie elektronová rastrovací metody MeSH
- spory hub účinky léků ultrastruktura MeSH
- thiazolidiny farmakologie MeSH
- tvar buňky účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- bicyklické sloučeniny heterocyklické MeSH
- latrunculin A MeSH Prohlížeč
- thiazolidiny MeSH
BACKGROUND: The aim of this basic study was to investigate by scanning electron microscopy the effects of cytoskeleton inhibitors on conidiogenesis and capsule in the yeast Fellomyces fuzhouensis CBS 8243, related to Cryptococcus neoformans. METHODS: Cells were treated by methyl benzimidazole-2-ylcarbamate (BCM) and latrunculin A (LAT) in yeast extract peptone dextrose medium and examined by scanning electron microscopy. RESULTS: During conidiogenesis, mother cells covered by capsule formed hypha-like stalks and at the hyphal tip yeast-like conidium developed. LAT blocked both stages of conidiogenesis. Inhibited mother cells and conidia became spherical and their capsule disappeared. BCM did not block formation of conidia that were neckless, or affect capsule. Combined application of LAT and BCM blocked both stages of conidiogenesis, cells became spherical and their capsule disappeared. CONCLUSION: Yeast cells with disrupted actin cytoskeleton do not reproduce by conidiogenesis and do not retain inherited cell shape and capsule.
Citace poskytuje Crossref.org
