Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20599238
DOI
10.1016/j.rvsc.2010.06.017
PII: S0034-5288(10)00224-9
Knihovny.cz E-resources
- MeSH
- Cell Death physiology MeSH
- Cells, Cultured MeSH
- Lipopolysaccharide Receptors genetics metabolism MeSH
- Mammary Glands, Animal physiology MeSH
- Neutrophils cytology metabolism MeSH
- Gene Expression Regulation physiology MeSH
- Cattle * MeSH
- Animals MeSH
- Check Tag
- Cattle * MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Lipopolysaccharide Receptors MeSH
This paper investigates the association between expression of CD14 and occurrence of apoptosis in blood, resident ((RES)PMN) and inflammatory ((INF)PMN) polymorphonuclear leukocytes (PMN) from heifer mammary glands. The fresh population of (RES)PMN contained a statistically significant higher proportion of CD14+, apoptotic and necrotic cells than did populations of (INF)PMN and blood PMN. In vitro cultivation of (RES)PMN, (INF)PMN and blood PMN led to concurrent increase of apoptotic, necrotic and CD14+ cells. A positive correlation was found between the proportions of both apoptotic and necrotic PMN and CD14+ PMN as determined by three-color flow cytometry analysis. Our study confirmed that expression of CD14 in blood PMN, (RES)PMN and (INF)PMN from heifer mammary glands was accompanied by apoptosis and necrosis.
References provided by Crossref.org
