Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20599238
DOI
10.1016/j.rvsc.2010.06.017
PII: S0034-5288(10)00224-9
Knihovny.cz E-zdroje
- MeSH
- buněčná smrt fyziologie MeSH
- kultivované buňky MeSH
- lipopolysacharidové receptory genetika metabolismus MeSH
- mléčné žlázy zvířat fyziologie MeSH
- neutrofily cytologie metabolismus MeSH
- regulace genové exprese fyziologie MeSH
- skot * MeSH
- zvířata MeSH
- Check Tag
- skot * MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- lipopolysacharidové receptory MeSH
This paper investigates the association between expression of CD14 and occurrence of apoptosis in blood, resident ((RES)PMN) and inflammatory ((INF)PMN) polymorphonuclear leukocytes (PMN) from heifer mammary glands. The fresh population of (RES)PMN contained a statistically significant higher proportion of CD14+, apoptotic and necrotic cells than did populations of (INF)PMN and blood PMN. In vitro cultivation of (RES)PMN, (INF)PMN and blood PMN led to concurrent increase of apoptotic, necrotic and CD14+ cells. A positive correlation was found between the proportions of both apoptotic and necrotic PMN and CD14+ PMN as determined by three-color flow cytometry analysis. Our study confirmed that expression of CD14 in blood PMN, (RES)PMN and (INF)PMN from heifer mammary glands was accompanied by apoptosis and necrosis.
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