Singlet oxygen scavenging activity of plastoquinol in photosystem II of higher plants: electron paramagnetic resonance spin-trapping study
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20637718
DOI
10.1016/j.bbabio.2010.07.003
PII: S0005-2728(10)00655-9
Knihovny.cz E-resources
- MeSH
- Cell Membrane drug effects metabolism MeSH
- Electron Spin Resonance Spectroscopy * MeSH
- Photosystem II Protein Complex chemistry metabolism MeSH
- Plastoquinone analogs & derivatives chemistry MeSH
- Free Radical Scavengers pharmacology MeSH
- Singlet Oxygen chemistry MeSH
- Spin Trapping MeSH
- Spinacia oleracea chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Photosystem II Protein Complex MeSH
- Plastoquinone MeSH
- plastoquinol MeSH Browser
- Free Radical Scavengers MeSH
- Singlet Oxygen MeSH
Singlet oxygen (¹O₂) scavenging activity of plastoquinol in photosystem II (PSII) of higher plants was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that illumination of spinach PSII membranes deprived of intrinsic plastoquinone results in ¹O₂ formation, as monitored by TEMPONE EPR signal. Interestingly, the addition of exogenous plastoquinol (PQH₂-1) to PQ-depleted PSII membranes significantly suppressed TEMPONE EPR signal. The presence of exogenous plastoquinols with a different side-chain length (PQH₂-n, n isoprenoid units in the side chain) caused a similar extent of ¹O₂ scavenging activity. These observations reveal that plastoquinol exogenously added to PQ-depleted PSII membranes serves as efficient scavenger of ¹O₂.
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