Singlet oxygen scavenging activity of plastoquinol in photosystem II of higher plants: electron paramagnetic resonance spin-trapping study

. 2010 Nov ; 1797 (11) : 1807-11. [epub] 20100715

Jazyk angličtina Země Nizozemsko Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid20637718
Odkazy

PubMed 20637718
DOI 10.1016/j.bbabio.2010.07.003
PII: S0005-2728(10)00655-9
Knihovny.cz E-zdroje

Singlet oxygen (¹O₂) scavenging activity of plastoquinol in photosystem II (PSII) of higher plants was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that illumination of spinach PSII membranes deprived of intrinsic plastoquinone results in ¹O₂ formation, as monitored by TEMPONE EPR signal. Interestingly, the addition of exogenous plastoquinol (PQH₂-1) to PQ-depleted PSII membranes significantly suppressed TEMPONE EPR signal. The presence of exogenous plastoquinols with a different side-chain length (PQH₂-n, n isoprenoid units in the side chain) caused a similar extent of ¹O₂ scavenging activity. These observations reveal that plastoquinol exogenously added to PQ-depleted PSII membranes serves as efficient scavenger of ¹O₂.

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