Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20664602
DOI
10.1038/ja.2010.93
PII: ja201093
Knihovny.cz E-resources
- MeSH
- Butyrates analysis MeSH
- Chromatography, Liquid methods MeSH
- Fermentation MeSH
- Hemiterpenes MeSH
- Spectrometry, Mass, Electrospray Ionization methods MeSH
- Isobutyrates MeSH
- Culture Media chemistry MeSH
- Pentanoic Acids analysis MeSH
- Macrolides chemistry isolation & purification metabolism MeSH
- Propionates analysis MeSH
- Streptomyces griseus growth & development metabolism radiation effects MeSH
- Ultraviolet Rays MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Butyrates MeSH
- Hemiterpenes MeSH
- Isobutyrates MeSH
- isobutyric acid MeSH Browser
- isovaleric acid MeSH Browser
- Culture Media MeSH
- Pentanoic Acids MeSH
- Macrolides MeSH
- nonactin MeSH Browser
- Propionates MeSH
Precursor-directed biogenetic approach was used to produce a range of nonactin homologues in a 50 l fermentor cultivation of strain Streptomyces griseus 34/249 obtained by UV mutagenesis. The production medium contained sodium propionate, isobutyrate and isovalerate as individual precursors, and 10 g l(-1) Diaion HP20 styrene-divinylbenzene resin that maintains suitable precursor concentration by reversibly adsorbing and releasing it. The produced nonactin homologues were separated on two C18 reversed-phase liquid chromatography columns in series and analyzed by MS with ESI source in the positive ion mode. Formation of doubly charged ions was suppressed by an excess of Na(+) ions throughout the process. The production of the homologues increased up to day 5 and then it leveled off. Cultivations with individual precursors yielded a total of 18 nonactin homologues whose spectrum depended on the precursor used. The total production of the homologues was lowered but their spectrum was shifted to higher-molecular-weight compounds.
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