Silica-based monolithic capillary columns-Effect of preparation temperature on separation efficiency
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20674924
DOI
10.1016/j.chroma.2010.07.010
PII: S0021-9673(10)00914-3
Knihovny.cz E-resources
- MeSH
- Benzene Derivatives chemistry MeSH
- Hydrogen-Ion Concentration MeSH
- Microscopy, Electron, Scanning MeSH
- Urea chemistry MeSH
- Silicon Dioxide chemistry MeSH
- Polyethylene Glycols chemistry MeSH
- Silanes chemistry MeSH
- Temperature MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Phase Transition MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Benzene Derivatives MeSH
- Urea MeSH
- Silicon Dioxide MeSH
- Polyethylene Glycols MeSH
- Silanes MeSH
- tetramethoxysilane MeSH Browser
The temperature effects during the sol-gel process and ageing of the silica-based monolith on the structure and separation efficiency of the capillary columns (100microm i.d., 150mm) for HPLC separations were studied. The tested columns were synthesized from a mixture of tetramethoxysilane, polyethylene glycol and urea under the acidic conditions. The temperature was varied from 40 degrees C to 44 degrees C and formation of bypass channels between the silica mold and the capillary wall was examined. The temperature of 43 degrees C was estimated as optimal for preparation of efficient silica capillary columns which were subsequently modified by octadecyldimethyl-N,N-diethylaminosilane or covered by poly(octadecyl methacrylate) and tested using standard mixture of alkylbenzenes under the isocratic conditions.
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