Tortoise tick Hyalomma aegyptium as long term carrier of Q fever agent Coxiella burnetii--evidence from experimental infection
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Coxiella burnetii izolace a purifikace MeSH
- Ixodidae mikrobiologie MeSH
- larva mikrobiologie MeSH
- morčata MeSH
- zdroje nemoci mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- morčata MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The experimental study investigated the ability of tortoise tick Hyalomma aegyptium to play a role in forming and maintaining natural foci of Q fever. We tested the competence of H. aegyptium larvae to acquire Coxiella burnetii infection from mammals, serve as a C. burnetii vector between mammalian hosts, and be a long-term carrier of C. burnetii, including interstadial transmission. H. aegyptium larvae were allowed to feed on guinea pigs experimentally infected with C. burnetii. Engorged larvae molted to nymphs, some of which were preserved in 96% ethanol and later examined by polymerase chain reaction (PCR) using C. burnetii-specific primers (CBCOS, CBCOE). Prevalence of C. burnetii among these nymphs was 5.6% (n=235). Remaining nymphs then fed on other, C. burnetii-negative guinea pigs; and according to results of both, micro-agglutination reaction, and ELISA, they successfully transmitted C. burnetii to those new hosts. Detached engorged nymphs molted to adults, which were kept alive long term and then placed in 96% ethanol 383 days post-infection. Thereafter, they were examined by PCR in the same manner as were the nymphs. Prevalence of C. burnetii among adult H. aegyptium was 28.9% (n=90). According to our results, tortoise-specific ticks have indisputable potential in the epidemiology of Q fever natural foci.
Zobrazit více v PubMed
Eur J Epidemiol. 2003;18(3):263-6 PubMed
J Parasitol. 2000 Jun;86(3):438-41 PubMed
Parasitol Today. 1998 May;14(5):186-92 PubMed
Am J Trop Med Hyg. 2003 Sep;69(3):314-7 PubMed
Parazitologiia. 2003 Jan-Feb;37(1):47-59 PubMed
J Wildl Dis. 1979 Jan;15(1):15-7 PubMed
Indian J Med Res. 1979 Dec;70:937-41 PubMed
Indian J Med Res. 1979 Feb;69:244-6 PubMed
Parasitol Res. 2008 Dec;103 Suppl 1:S109-16 PubMed
Bull Soc Pathol Exot Filiales. 1959 Sep-Oct;52:564-7 PubMed
J Parasitol. 2000 Oct;86(5):1135-6 PubMed
Clin Infect Dis. 2001 Mar 15;32(6):897-928 PubMed
Acta Virol. 1969 Jan;13(1):60-6 PubMed
Infect Immun. 1993 Feb;61(2):470-7 PubMed
Vector Borne Zoonotic Dis. 2009 Jun;9(3):331-6 PubMed
Clin Lab. 2000;46(5-6):239-45 PubMed
Int J Zoonoses. 1980 Dec;7(2):85-9 PubMed
J Bacteriol. 1989 Aug;171(8):4202-6 PubMed
Parasite. 2009 Dec;16(4):259-73 PubMed
Exp Appl Acarol. 1996 Jan;20(1):23-30 PubMed
Bull World Health Organ. 1968;39(6):974-7 PubMed
J Vet Med Sci. 1998 Jul;60(7):781-90 PubMed
Emerg Infect Dis. 2006 Dec;12(12):1895-901 PubMed
C R Hebd Seances Acad Sci. 1960 Jun 20;250:4225-6 PubMed
Acta Virol. 1987 May;31(3):254-9 PubMed
Annu Rev Entomol. 1967;12:347-76 PubMed
Int J Antimicrob Agents. 1997;8(3):145-61 PubMed
Exp Appl Acarol. 2006;40(3-4):279-90 PubMed
Zoonotic Parasites of Reptiles: A Crawling Threat
Transstadial transmission of Borrelia turcica in Hyalomma aegyptium ticks
Life cycle of tortoise tick Hyalomma aegyptium under laboratory conditions