Borrelia turcica comprises the third major group of arthropod-transmitted borreliae and is phylogenetically divergent from other Borrelia groups. The novel group of Borrelia was initially isolated from Hyalomma aegyptium ticks in Turkey and it was recently found in blood and multiple organs of tortoises exported from Jordan to Japan. However, the ecology of these spirochetes and their development in ticks or the vertebrate hosts were not investigated in detail; our aims were to isolate the pathogen and to evaluate the possibility of transstadial transmission of Borrelia turcica by H. aegyptium ticks. Ticks were collected from Testudo graeca tortoises during the summer of 2013 from southeastern Romania. Engorged nymphs were successfully molted to the adult stage. Alive B. turcica was isolated from molted ticks by using Barbour-Stoenner-Kelly (BSK) II medium. Four pure cultures of spirochetes were obtained and analyzed by PCR and sequencing. Sequence analysis of glpQ, gyrB and flaB revealed 98%-100% similarities with B. turcica. H. aegyptium ticks collected from T. graeca tortoises were able to pass the infection with B. turcica via transstadial route, suggesting its vectorial capacity.

Department of Parasitology and Parasitic Diseases University of Agricultural Sciences and Veterinary Medicine Cluj Napoca Cluj Napoca Romania

Department of Pathology and Parasitology Faculty of Veterinary Medicine University of Veterinary and Pharmaceutical Sciences Brno Czech Republic; Biology Centre Institute of Parasitology Czech Academy of Sciences České Budějovice Czech Republic

National Institute of Public Health and Environment Bilthoven the Netherlands

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Orkun Ö, Karaer Z, Çakmak A, Nalbantoğlu S (2014) Identification of tick-borne pathogens in ticks feeding on humans in Turkey. PLoS Negl Trop Dis 8: e3067 10.1371/journal.pntd.0003067 PubMed DOI PMC

Rar VA, Fomenko NV, Dobrotvorsky AK, Livanova NN, Rudakova SA, et al. (2005) Tickborne pathogen detection, Western Siberia, Russia. Emerg Infect Dis 11: 1708–1715. PubMed PMC

Dantas-Torres F, Chomel BB, Otranto D (2012) Ticks and tick-borne diseases: a One Health perspective. Trends Parasitol 28: 437–446. 10.1016/j.pt.2012.07.003 PubMed DOI

Takano A, Sugimori C, Fujita H, Kadosaka T, Taylor KR, et al. (2012) A novel relapsing fever PubMed DOI

Franke J, Hildebrandt A, Dorn W (2013) Exploring gaps in our knowledge on Lyme borreliosis spirochaetes—updates on complex heterogeneity, ecology, and pathogenicity. Ticks Tick Borne Dis 4: 11–25. 10.1016/j.ttbdis.2012.06.007 PubMed DOI

Gern L, Humair PF (1998) Natural history of PubMed

Stanek G, Reiter M (2011) The expanding Lyme PubMed DOI

Barbour AG, Putteet-Driver AD, Bunikis J (2005) Horizontally acquired genes for purine salvage in PubMed PMC

Banerjee SN, Banerjee M, Fernando K, Burgdorfer W, Schwan TG (1998) Tick-borne relapsing fever in British Columbia, Canada: first isolation of PubMed PMC

van Dam AP, van Gool T, Wetsteyn JC, Dankert J (1999) Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat analysis and PubMed PMC

Elbir H, Raoult D, Drancourt M (2013) Relapsing fever borreliae in Africa. Am J Trop Med Hyg 89: 288–292. 10.4269/ajtmh.12-0691 PubMed DOI PMC

Al-Gwaiz LA, Al-Mashhadani SA, Ayoola EA, Al-Khairy KS, Higgy KG, et al. (1995) Relapsing fever in Saudi Arabia: Report of two cases. Ann Saudi Med 15: 165–167. PubMed

Dupont HT, La Scola B, Williams R, Raoult D (1997) A focus of tick-borne relapsing fever in southern Zaire. Clin Infect Dis 25: 139–144. PubMed

Rizzoli A, Hauffe H, Carpi G, Vourc HG, Neteler M, et al. (2011) Lyme borreliosis in Europe. Euro Surveill 7: 16. PubMed

Richter D, Schlee DB, Matuschka FR (2003) Relapsing fever-like spirochetes infecting European vector tick of Lyme disease agent. Emerg Infect Dis 9: 697–701. PubMed PMC

Masuzawa T (2004) Terrestrial distribution of the Lyme borreliosis agent PubMed

Burridge MJ (2011) Invasive species In: Non-native and invasive ticks. Threats to human and animal health in the United States. University press of Florida; pp. 4–14.

Burridge MJ, Simmons LA, Allan SA (2000) Introduction of potential heart water vectors and other exotic ticks into Florida on imported reptiles. J Parasitol 86: 700–704. PubMed

Burridge MJ, Simmons LA (2003) Exotic ticks introduced into the United States on imported reptiles from 1962 to 2001 and their potential roles in international dissemination of diseases. Vet Parasitol 113: 289–320. PubMed

Paperna I, Kremer-Mecabell T, Finkelman S (2002) PubMed

Güner ES, Watanabe M, Hashimoto N, Kadosaka T, Kawamura Y, et al. (2004) PubMed

Bitam I, Kernif T, Harrat Z, Parola P, Raoult D (2009) First detection of PubMed DOI

Takano A, Goka K, Une Y, Shimada Y, Fujita H, et al. (2010) Isolation and characterization of a novel PubMed DOI

Harris DJ, Maia JP, Perera A (2011) Molecular characterization of Hepatozoon species in reptiles from the Seychelles. J Parasitol 97: 106–110. 10.1645/GE-2470.1 PubMed DOI

Paștiu AI, Matei IA, Mihalca AD, D’Amico G, Dumitrache MO, et al. (2012) Zoonotic pathogens associated with PubMed DOI PMC

Güner ES, Hashimoto N, Takada N, Kaneda K, Imai Y, et al. (2003) First isolation and characterization of PubMed

Takano A, Fujita H, Kadosaka T, Konnai S, Tajima T, Watanabe H, Ohnishi M, Kawabata H (2011) Characterization of reptile-associated PubMed DOI

Adeolu M, Gupta RS (2014) A phylogenomic and molecular marker based proposal for the division of the genus PubMed

Schwan TG, Anderson JM, Lopez JE, Fischer RJ, Raffel SJ, et al. (2012) Endemic foci of the tick-borne relapsing fever spirochete PubMed DOI PMC

Cutler SJ (2006) Possibilities for relapsing fever reemergence. Emerg Infect Dis 12: 369–374. PubMed PMC

Ray HN (1950) Hereditary transmission of PubMed

Kar S, Yılmazer N, Midilli K, Ergin S, Alp H, et al. (2011) Presence of the zoonotic

Široký P, Mikulícek P, Jandzík D, Kami H, Mihalca AD, et al. (2009) Co-distribution pattern of a haemogregarine PubMed DOI

Široký P, Kubelová M, Modrý D, Erhart J, Literák I, et al. (2010) Tortoise tick PubMed DOI

Široký P, Bělohlávek T, Papoušek I, Jandzik D, Mikulíček P, et al. (2014) Hidden threat of tortoise ticks: high prevalence of Crimean-Congo haemorrhagic fever virus in ticks PubMed PMC

Vatansever Z, Gargili A, Aysul NS, Sengoz G, Estrada-Peña A (2008) Ticks biting humans in the urban area of Istanbul. Parasitol Res 102: 551–553. PubMed

Bursali A, Tekin S, Orhan M, Keskin A, Ozkan M (2010) Ixodid ticks (Acari: Ixodidae) infesting humans in Tokat Province of Turkey: species diversity and seasonalactivity. J Vector Ecol 35: 180–186. 10.1111/j.1948-7134.2010.00045.x PubMed DOI

Kar S, Dervis E, Akın A, Ergonul O, Gargili A (2013) Preferences of different tick species for human hosts in Turkey. Exp Appl Acarol 61: 349–355. 10.1007/s10493-013-9698-2 PubMed DOI

Apanaskevich DA: Host–parasite relationships of the genus Hyalomma Koch (Acari, Ixodidae) and their connection with microevolutionary processes. Parazitologiya 2004, 38:515–523. in Russian PubMed

Siroký P,Erhart J, Petrželková KJ, Kamler M. (2011). Life cycle of tortoise tick PubMed DOI

Sanders FH, Oliver JH (1995) Evaluation of PubMed

Dolan MC, Maupin GO, Panella NA, Golde WT, Piesman J (1997) Vector competence of PubMed

Eisen L, Lane RS (2002) Vectors of

Feider Z (1965) Fauna RPR. Arachnida In romanian. Bucureşti: Academiei RPR, vol V, fasc 2.

Postic D, Assous MV, Grimont PA, Baranton G (1994) Diversity of PubMed

Schwan TG, Raffael JS, Schrumpf ME, Policastro PF, Rawlings JA, et al. (2005) Phylogenetic analysis of the spirochetes PubMed PMC

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molec Biol Evol 30: 2725–2729. 10.1093/molbev/mst197 PubMed DOI PMC

Saitou N, Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molec Biol Evol 4:406–425. PubMed

Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783–791. PubMed

Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030–11035. PubMed PMC

Kolonin GV (1983) Mirovoe rasprostranenie iksodovykh kleshchey Rody

Mihalca AD, Dumitrache MO, Magdaş C, Gherman CM, Domşa C, et al. (2012) Synopsis of the hard ticks (Acari: Ixodidae) of Romania with update on host associations and geographical distribution. Exp Appl Acarol 58: 183–206. 10.1007/s10493-012-9566-5 PubMed DOI

Kalmár Z, D’Amico G, Matei IA, Paștiu AI, Mărcuţan DI, et al. (2014)

Bacon RM, Pilgard MA, Johnson BJ, Raffel SJ, Schwan TG (2004) Glycerophosphodiester phosphodiesterase gene ( PubMed PMC

Ticks and tick-borne pathogens in wild birds in Greece