Fluorescent in situ hybridization of mitochondrial DNA and RNA
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21125028
PII: 20102044
Knihovny.cz E-resources
- MeSH
- In Situ Hybridization, Fluorescence * MeSH
- Microscopy, Confocal MeSH
- Humans MeSH
- DNA, Mitochondrial metabolism MeSH
- Cell Line, Tumor MeSH
- RNA, Mitochondrial MeSH
- RNA metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Mitochondrial MeSH
- RNA, Mitochondrial MeSH
- RNA MeSH
To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by the light strand promoter minor RNA. Confocal microscopy demonstrated ND5 probe spreading (less for other probes) in mitochondrial reticulum tubules but upon RNase A treatment all probes contoured mtDNA nucleoid localization. DNase I spread the signal over mitochondrial tubules. Future applications are discussed.
