Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single-stranded and double-stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several different crystallization conditions. The presence of Zn(2+) ions was confirmed by X-ray fluorescence. First crystallographic data were obtained.

Institute of Macromolecular Chemistry AS CR v v i Heyrovského nám 2 162 06 Praha 6 Czech Republic

Zobrazit více v PubMed

Alexsandrowicz, J. (1958). Lancet, 2, 420.

Better, M., Bernhard, S., Lei, S. P., Fishwild, D. & Carroll, S. (1992). J. Biol. Chem.267, 16712–16718. PubMed

Chang, S. C. & Gallie, D. R. (1997). Plant Physiol.113, 1253–1263. PubMed PMC

D’Alessio, G. & Leone, E. (1963). Biochem. J.89, 183.

Dangl, J. L., Dietrich, R. A. & Thomas, H. (2000). Biochemistry and Molecular Biology of Plants, edited by B. Buchman, W. Gruissen and R. Jones, pp. 1044–1100. Rockville: American Society of Plant Physiology.

Darzynkiewicz, Z., Carter, S. P., Mikulski, S. M., Ardelt, W. J. & Shogen, K. (1988). Cell Tissue Kinet.21, 169–182. PubMed

Deonarain, M. P. & Epenetos, A. A. (1998). Br. J. Cancer, 77, 537–546. PubMed PMC

Koval, T., Dohnalek, J., Lipovova, P., Podzimek, T. & Matousek, J. (2009). WDS’09 Proceedings of Contributed Papers, Part III – Physics, edited by J. Safrankova and J. Pavlu, pp. 193–196. Prague: MATFYZPRESS.

Ledoux, L. (1955a). Nature (London), 175, 258–259. PubMed

Ledoux, L. (1955b). Nature (London), 176, 36–37. PubMed

Ledoux, L. & Baltus, E. (1954). Experientia, 10, 500–501. PubMed

Matousek, J. (1973). Experientia, 29, 858–859. PubMed

Matousek, J. (1975). J. Reprod. Fert.43, 171–174. PubMed

Matousek, J. (2001). Comput. Biochem. Physiol. C, 129, 175–191. PubMed

Matousek, J., Kozlova, P., Orctova, L., Schmitz, A., Pesina, K., Bannach, O., Diermann, N., Steger, G. & Riesner, D. (2007). Biol. Chem.388, 1–13. PubMed

Matousek, J. & Matousek, J. (2010). Rec. Pat. DNA Gene Seq.4, 29–39. PubMed

Matousek, J., Podzimek, T., Pouckova, P., Stehlik, J., Skvor, J., Lipovova, P. & Matousek, J. (2010). Neoplasma, 57, 339–348. PubMed

Matousek, J., Soucek, J., Slavik, T., Tomanek, M., Lee, J. E. & Raines, R. T. (2003). Comput. Biochem. Physiol. C, 136, 343–356. PubMed

Otwinowski, Z. & Minor, W. (1997). Methods Enzymol.276, 307–326. PubMed

Sheldrick, G. M. (2008). Acta Cryst. A64, 112–122. PubMed

Soucek, J., Marinov, I., Benes, J., Hilgert, I., Matousek, J. & Raines, R. T. (1996). Immunobiology, 195, 271–285. PubMed

Wool, I. G. (1997). Ribonucleases: Structures and Functions, edited by G. D’Alessio & J. F. Riordan, pp. 131–162. New York: Academic Press.

Phosphate binding in the active centre of tomato multifunctional nuclease TBN1 and analysis of superhelix formation by the enzyme