Accumulation of viroid-specific small RNAs and increase in nucleolytic activities linked to viroid-caused pathogenesis
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17214544
DOI
10.1515/bc.2007.001
Knihovny.cz E-resources
- MeSH
- Biolistics methods MeSH
- Endonucleases genetics metabolism MeSH
- Genotype MeSH
- Cloning, Molecular MeSH
- DNA, Complementary chemistry genetics MeSH
- Nucleic Acid Conformation MeSH
- Plant Leaves genetics metabolism virology MeSH
- RNA, Small Interfering genetics metabolism MeSH
- Molecular Sequence Data MeSH
- Plant Diseases genetics virology MeSH
- Blotting, Northern MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- RNA, Viral chemistry genetics metabolism MeSH
- Plant Proteins genetics metabolism MeSH
- Plant Viruses genetics pathogenicity MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology, Amino Acid MeSH
- Solanum lycopersicum genetics metabolism virology MeSH
- Solanum tuberosum genetics metabolism virology MeSH
- Viroids genetics pathogenicity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Endonucleases MeSH
- DNA, Complementary MeSH
- RNA, Small Interfering MeSH
- RNA, Viral MeSH
- Plant Proteins MeSH
Strong viroid-caused pathogenesis was achieved in tomato cv. Rutgers by biolistic transfer of severe or lethal potato spindle tuber viroid (PSTVd) strains, while other tomato genotypes (e.g., Moneymaker) were tolerant. With reciprocal hybrids between sensitive and tolerant genotypes, we show that plant depression dominates over tolerance. Biolistic transfer of the most pathogenic PSTVd strain AS1 to Nicotiana benthamiana, which is considered to be a symptomless PSTVd host, led to a strong pathogenesis reaction and stunting, suggesting the presence of specific viroid pathogenesis-promoting target(s) in this plant species. Total levels of small siRNA-like PSTVd-specific RNAs were enhanced in strongly symptomatic tomato and N. benthamiana plants after biolistic infection with AS1 in comparison to the mild QFA strain. This indicates association of elevated levels of viroid-specific small RNA with production of strong symptoms. In symptom-bearing tomato leaves in comparison to controls, an RNase of approximately 18 kDa was induced and the activity of a nuclease of 34 kDa was elevated by a factor of seven in the vascular system. Sequence analysis of the nuclease cDNA designated TBN1 showed high homology with plant apoptotic endonucleases. The vascular-specific pathogenesis action is supported by light microscopic observations demonstrating a certain lack of xylem tissue and an arrest of the establishment of new vascular bundles in collapsed plants.
References provided by Crossref.org
Viroid quasispecies revealed by deep sequencing
Crystallization of recombinant bifunctional nuclease TBN1 from tomato
Structure analysis of group I plant nucleases
GENBANK
AM238701
