Macrophage gene expression is related to obesity and the metabolic syndrome in human subcutaneous fat as well as in visceral fat
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adult MeSH
- Cells, Cultured MeSH
- Middle Aged MeSH
- Humans MeSH
- Macrophages metabolism MeSH
- Metabolic Syndrome metabolism MeSH
- Young Adult MeSH
- Overweight metabolism MeSH
- Intra-Abdominal Fat cytology metabolism MeSH
- Obesity metabolism MeSH
- Subcutaneous Fat cytology metabolism MeSH
- Aged MeSH
- Adipose Tissue cytology metabolism MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
AIMS/HYPOTHESIS: Our goal was to identify a set of human adipose tissue macrophage (ATM)-specific markers and investigate whether their gene expression in subcutaneous adipose tissue (SAT) as well as in visceral adipose tissue (VAT) is related to obesity and to the occurrence of the metabolic syndrome. METHODS: ATM-specific markers were identified by DNA microarray analysis of adipose tissue cell types isolated from SAT of lean and obese individuals. We then analysed gene expression of these markers by reverse transcription quantitative PCR in paired samples of SAT and VAT from 53 women stratified into four groups (lean, overweight, obese and obese with the metabolic syndrome). Anthropometric measurements, euglycaemic-hyperinsulinaemic clamp, blood analysis and computed tomography scans were performed. RESULTS: A panel of 24 genes was selected as ATM-specific markers based on overexpression in ATM compared with other adipose tissue cell types. In SAT and VAT, gene expression of ATM markers was lowest in lean and highest in the metabolic syndrome group. mRNA levels in the two fat depots were negatively correlated with glucose disposal rate and positively associated with indices of adiposity and the metabolic syndrome. CONCLUSIONS/INTERPRETATION: In humans, expression of ATM-specific genes increases with the degree of adiposity and correlates with markers of insulin resistance and the metabolic syndrome to a similar degree in SAT and in VAT.
See more in PubMed
Diabetes. 2010 Jul;59(7):1648-56 PubMed
Presse Med. 1947 May 24;55(30):339 PubMed
J Lipid Res. 2009 Apr;50 Suppl:S282-6 PubMed
Metabolism. 1987 Jan;36(1):54-9 PubMed
Diabetes Care. 1999 Sep;22(9):1471-8 PubMed
J Clin Endocrinol Metab. 2008 Aug;93(8):3215-21 PubMed
Arterioscler Thromb Vasc Biol. 2009 Oct;29(10):1608-14 PubMed
J Inflamm (Lond). 2009 Mar 16;6:6 PubMed
Hum Genet. 2003 May;112(5-6):581-92 PubMed
Diabetologia. 2008 Dec;51(12):2303-8 PubMed
Circulation. 2008 Feb 12;117(6):806-15 PubMed
Int J Obes (Lond). 2007 Sep;31(9):1420-8 PubMed
FASEB J. 2004 Nov;18(14):1657-69 PubMed
Diabetes Metab. 2009 Sep;35(4):251-60 PubMed
Diabetes. 2009 Feb;58(2):307-8 PubMed
Int J Obes (Lond). 2011 Jan;35(1):91-8 PubMed
J Clin Invest. 2007 Jan;117(1):175-84 PubMed
Obes Surg. 2009 Nov;19(11):1564-73 PubMed
Lancet. 2005 Sep 24-30;366(9491):1059-62 PubMed
PLoS One. 2010 Feb 23;5(2):e9369 PubMed
Diabetes. 2005 Aug;54(8):2277-86 PubMed
J Clin Endocrinol Metab. 2007 Jun;92(6):2240-7 PubMed
J Lipid Res. 2005 Nov;46(11):2347-55 PubMed
Int J Obes (Lond). 2009 Sep;33(9):978-90 PubMed
Circulation. 2004 Jul 20;110(3):349-55 PubMed
Nat Rev Immunol. 2008 Dec;8(12):958-69 PubMed
PLoS One. 2008 Mar 05;3(3):e1713 PubMed
Nat Immunol. 2010 May;11(5):373-84 PubMed
J Leukoc Biol. 2000 Jan;67(1):97-103 PubMed
Nat Med. 2009 Apr;15(4):373-4 PubMed
Curr Pharm Des. 2007;13(21):2148-68 PubMed
J Immunol. 2006 Nov 15;177(10):7303-11 PubMed
Arch Intern Med. 2005 Apr 11;165(7):777-83 PubMed
Diabetes. 2009 Feb;58(2):385-93 PubMed
Diabetes. 2006 Jun;55(6):1554-61 PubMed
Diabetes. 2008 Dec;57(12):3239-46 PubMed
Am J Physiol. 1979 Sep;237(3):E214-23 PubMed
Obes Res. 2004 Aug;12(8):1217-22 PubMed
Nat Genet. 2003 Jul;34(3):267-73 PubMed
Nature. 2008 Jun 5;453(7196):783-7 PubMed
Circulation. 2007 Jul 3;116(1):39-48 PubMed
FASEB J. 2004 Jan;18(1):215-7 PubMed
Int J Obes (Lond). 2009 Nov;33(11):1257-64 PubMed
Diabetes. 2009 Jul;58(7):1558-67 PubMed
Diabetes. 2004 May;53(5):1285-92 PubMed
Expert Rev Cardiovasc Ther. 2008 Mar;6(3):343-68 PubMed
J Clin Invest. 2003 Dec;112(12):1796-808 PubMed
Arterioscler Thromb Vasc Biol. 2008 Jun;28(6):1039-49 PubMed
Diabetes Care. 2009 Mar;32(3):481-5 PubMed
Serum oxLDL-β2GPI complex reflects metabolic syndrome and inflammation in adipose tissue in obese
