Dietary intervention-induced weight loss decreases macrophage content in adipose tissue of obese women
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20531347
DOI
10.1038/ijo.2010.112
PII: ijo2010112
Knihovny.cz E-zdroje
- MeSH
- bílá tuková tkáň patologie MeSH
- C-reaktivní protein genetika MeSH
- chemokin CXCL5 genetika MeSH
- dospělí MeSH
- down regulace MeSH
- hmotnostní úbytek * genetika MeSH
- index tělesné hmotnosti MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé MeSH
- longitudinální studie MeSH
- makrofágy patologie MeSH
- obezita dietoterapie genetika patologie MeSH
- průtoková cytometrie MeSH
- redukční dieta * MeSH
- stanovení celkové genové exprese MeSH
- tělesná hmotnost MeSH
- vezikulární transportní proteiny genetika MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- C-reaktivní protein MeSH
- chemokin CXCL5 MeSH
- CXCL5 protein, human MeSH Prohlížeč
- LYVE1 protein, human MeSH Prohlížeč
- vezikulární transportní proteiny MeSH
OBJECTIVE: Accumulation of adipose tissue macrophages (ATMs) is observed in obesity and may participate in the development of insulin resistance and obesity-related complications. The aim of our study was to investigate the effect of long-term dietary intervention on ATM content in human adipose tissue. DESIGN: We performed a multi-phase longitudinal study. SUBJECTS AND MEASUREMENTS: A total of 27 obese pre-menopausal women (age 39 ± 2 years, body mass index 33.7 ± 0.5 kg m(-2)) underwent a 6-month dietary intervention consisting of two periods: 4 weeks of very low-calorie diet (VLCD) followed by weight stabilization composed of 2 months of low-calorie diet and 3 to 4 months of weight maintenance diet. At baseline and at the end of each dietary period, samples of subcutaneous adipose tissue (SAT) were obtained by needle biopsy and blood samples were drawn. ATMs were determined by flow cytometry using combinations of cell surface markers. Selected cytokine and chemokine plasma levels were measured using enzyme-linked immunosorbent assay. In addition, in a subgroup of 16 subjects, gene expression profiling of macrophage markers in SAT was performed using real-time PCR. RESULTS: Dietary intervention led to a significant decrease in body weight, plasma insulin and C-reactive protein levels. After VLCD, ATM content defined by CD45+/14+/206+ did not change, whereas it decreased at the end of the intervention. This decrease was associated with a downregulation of macrophage marker mRNA levels (CD14, CD163, CD68 and LYVE-1 (lymphatic vessel endothelial hyaluronan receptor-1)) and plasma levels of monocyte-chemoattractant protein-1 (MCP-1) and CXCL5 (chemokine (C-X-C motif) ligand 5). During the whole dietary intervention, the proportion of two ATM subpopulations distinguished by the CD16 marker was not changed. CONCLUSION: A 6-month weight-reducing dietary intervention, but not VLCD, promotes a decrease in the number of the whole ATM population with no change in the relative distribution of ATM subsets.
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