Dietary intervention-induced weight loss decreases macrophage content in adipose tissue of obese women
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20531347
DOI
10.1038/ijo.2010.112
PII: ijo2010112
Knihovny.cz E-resources
- MeSH
- Adipose Tissue, White pathology MeSH
- C-Reactive Protein genetics MeSH
- Chemokine CXCL5 genetics MeSH
- Adult MeSH
- Down-Regulation MeSH
- Weight Loss * genetics MeSH
- Body Mass Index MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Humans MeSH
- Longitudinal Studies MeSH
- Macrophages pathology MeSH
- Obesity diet therapy genetics pathology MeSH
- Flow Cytometry MeSH
- Diet, Reducing * MeSH
- Gene Expression Profiling MeSH
- Body Weight MeSH
- Vesicular Transport Proteins genetics MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- C-Reactive Protein MeSH
- Chemokine CXCL5 MeSH
- CXCL5 protein, human MeSH Browser
- LYVE1 protein, human MeSH Browser
- Vesicular Transport Proteins MeSH
OBJECTIVE: Accumulation of adipose tissue macrophages (ATMs) is observed in obesity and may participate in the development of insulin resistance and obesity-related complications. The aim of our study was to investigate the effect of long-term dietary intervention on ATM content in human adipose tissue. DESIGN: We performed a multi-phase longitudinal study. SUBJECTS AND MEASUREMENTS: A total of 27 obese pre-menopausal women (age 39 ± 2 years, body mass index 33.7 ± 0.5 kg m(-2)) underwent a 6-month dietary intervention consisting of two periods: 4 weeks of very low-calorie diet (VLCD) followed by weight stabilization composed of 2 months of low-calorie diet and 3 to 4 months of weight maintenance diet. At baseline and at the end of each dietary period, samples of subcutaneous adipose tissue (SAT) were obtained by needle biopsy and blood samples were drawn. ATMs were determined by flow cytometry using combinations of cell surface markers. Selected cytokine and chemokine plasma levels were measured using enzyme-linked immunosorbent assay. In addition, in a subgroup of 16 subjects, gene expression profiling of macrophage markers in SAT was performed using real-time PCR. RESULTS: Dietary intervention led to a significant decrease in body weight, plasma insulin and C-reactive protein levels. After VLCD, ATM content defined by CD45+/14+/206+ did not change, whereas it decreased at the end of the intervention. This decrease was associated with a downregulation of macrophage marker mRNA levels (CD14, CD163, CD68 and LYVE-1 (lymphatic vessel endothelial hyaluronan receptor-1)) and plasma levels of monocyte-chemoattractant protein-1 (MCP-1) and CXCL5 (chemokine (C-X-C motif) ligand 5). During the whole dietary intervention, the proportion of two ATM subpopulations distinguished by the CD16 marker was not changed. CONCLUSION: A 6-month weight-reducing dietary intervention, but not VLCD, promotes a decrease in the number of the whole ATM population with no change in the relative distribution of ATM subsets.
References provided by Crossref.org
Serum oxLDL-β2GPI complex reflects metabolic syndrome and inflammation in adipose tissue in obese
