Soil and plant contamination with Mycobacterium avium subsp. paratuberculosis after exposure to naturally contaminated mouflon feces
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Feces microbiology MeSH
- Mycobacterium avium subsp. paratuberculosis genetics isolation & purification MeSH
- Sheep Diseases microbiology MeSH
- Sheep, Domestic * MeSH
- Paratuberculosis microbiology MeSH
- Soil chemistry MeSH
- Soil Microbiology * MeSH
- Plants microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Soil MeSH
The aim of this study was to demonstrate the persistence of Mycobacterium avium subsp. paratuberculosis (MAP) in soil and colonization of different plant parts after deliberate exposure to mouflon feces naturally contaminated with different amounts of MAP. Samples of aerial parts of plants, their roots, and the soil below the roots were collected after 15 weeks and examined using IS900 real-time quantitative PCR (qPCR) and cultivation. Although the presence of viable MAP cells was not demonstrated, almost all samples were found to be positive using qPCR. MAP IS900 was not only found in the upper green parts, but also in the roots and soil samples (from 1.00 × 10(0) to 6.43 × 10(3)). The level of soil and plant contamination was influenced mainly by moisture, clay content, and the depth from which the samples were collected, rather than by the initial concentration of MAP in the feces at the beginning of the experiment.
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Prev Vet Med. 2009 May 1;89(1-2):110-20 PubMed
Prev Vet Med. 1999 Jun 11;40(3-4):179-92 PubMed
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Appl Environ Microbiol. 2005 Sep;71(9):5304-8 PubMed
Vet Microbiol. 2000 Dec 20;77(3-4):283-90 PubMed
Nord Vet Med. 1977 Jun;29(6):267-70 PubMed
Appl Anim Behav Sci. 2000 Aug 1;69(1):15-33 PubMed
Can J Microbiol. 1984 Sep;30(9):1112-7 PubMed
J Appl Microbiol. 1997 Jan;82(1):57-67 PubMed
Microbiol Mol Biol Rev. 1997 Jun;61(2):121-35 PubMed
Vet Microbiol. 2000 Dec 20;77(3-4):291-7 PubMed
J Food Prot. 2008 Feb;71(2):397-401 PubMed
J Dairy Sci. 2004 Sep;87(9):2959-66 PubMed
Prev Vet Med. 2009 Jan 1;88(1):1-14 PubMed
J Dairy Sci. 2006 Mar;89(3):963-70 PubMed
Can J Vet Res. 2005 Apr;69(2):81-7 PubMed
Appl Environ Microbiol. 2004 May;70(5):2989-3004 PubMed
J Clin Microbiol. 2009 Mar;47(3):614-22 PubMed
Appl Environ Microbiol. 2009 Sep;75(17):5581-5 PubMed
J Clin Microbiol. 1998 Feb;36(2):402-8 PubMed
J Wildl Dis. 2002 Jul;38(3):629-32 PubMed
Can Vet J. 2008 Jun;49(6):569-76 PubMed
Appl Environ Microbiol. 2002 Jul;68(7):3639-43 PubMed
Appl Environ Microbiol. 2002 Jan;68(1):397-400 PubMed
Berl Munch Tierarztl Wochenschr. 2005 Jul-Aug;118(7-8):314-20 PubMed
