The effect of very-low-calorie diet on mRNA expression of inflammation-related genes in subcutaneous adipose tissue and peripheral monocytes of obese patients with type 2 diabetes mellitus
Language English Country United States Media print-electronic
Document type Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
PubMed
21289263
DOI
10.1210/jc.2010-1858
PII: jc.2010-1858
Knihovny.cz E-resources
- MeSH
- Adipokines genetics metabolism MeSH
- Chemokines genetics metabolism MeSH
- Diabetes Mellitus, Type 2 blood complications diet therapy genetics MeSH
- Caloric Restriction * MeSH
- Middle Aged MeSH
- Humans MeSH
- Inflammation Mediators metabolism MeSH
- RNA, Messenger genetics metabolism MeSH
- Monocytes metabolism MeSH
- Obesity blood complications diet therapy genetics MeSH
- Subcutaneous Fat metabolism MeSH
- Receptors, Adipokine genetics metabolism MeSH
- Receptors, Chemokine genetics metabolism MeSH
- Diet, Reducing MeSH
- Gene Expression Regulation * MeSH
- Inflammation complications genetics metabolism MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Controlled Clinical Trial MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adipokines MeSH
- Chemokines MeSH
- Inflammation Mediators MeSH
- RNA, Messenger MeSH
- Receptors, Adipokine MeSH
- Receptors, Chemokine MeSH
CONTEXT: Low-grade inflammation links obesity, type 2 diabetes mellitus (T2DM), and cardiovascular diseases. OBJECTIVE: To explore the expression profile of genes involved in inflammatory pathways in adipose tissue and peripheral monocytes (PM) of obese patients with and without T2DM at baseline and after dietary intervention. DESIGN: Two-week intervention study with very-low-calorie diet (VLCD). SETTING: University hospital. PATIENTS: Twelve obese females with T2DM, 8 obese nondiabetic females (OB) and 15 healthy age-matched females. INTERVENTION: Two weeks of VLCD (2500 kJ/d). MAIN OUTCOME MEASURES: Metabolic parameters, circulating cytokines, hormones, and mRNA expression of 39 genes in sc adipose tissue (SCAT) and PM. RESULTS: Both T2DM and OB group had significantly increased serum concentrations of circulating proinflammatory factors (C-reactive protein, TNFα, IL-6, IL-8), mRNA expression of macrophage antigen CD68 and proinflammatory chemokines (CCL-2, -3, -7, -8, -17, -22) in SCAT and complementary chemokine receptors (CCR-1, -2, -3, -5) and other proinflammatory receptors (toll-like receptor 2 and 4, TNF receptor superfamily 1A and 1B, IL-6R) in PM, with OB group showing less pronounced chemoattracting and proinflammatory profile compared to T2DM group. In T2DM patients VLCD decreased body weight, improved metabolic profile, and decreased mRNA expression of up-regulated CCRs in PM and chemokines [CCL 8, chemokine (C-X-C motif) ligand 10] in SCAT. VLCD markedly increased mRNA expression of T-lymphocyte attracting chemokine CCL-17 in SCAT. CONCLUSION: Obese patients with and without T2DM have increased mRNA expression of chemotactic and proinflammatory factors in SCAT and expression of corresponding receptors in PM. Two weeks of VLCD significantly improved this profile in T2DM patients.
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