Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- biologické modely MeSH
- difuze MeSH
- fluorescenční spektrometrie metody MeSH
- kationické antimikrobiální peptidy chemie metabolismus MeSH
- lipidové dvojvrstvy chemie metabolismus MeSH
- lipidy chemie MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kationické antimikrobiální peptidy MeSH
- lipidové dvojvrstvy MeSH
- lipidy MeSH
Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are used to detect changes in lipid and peptide diffusion upon addition of unlabelled arenicin-1 to SLBs. Arenicin-1 decreases lipid mobility in negatively charged SLBs. According to diffusion law analysis, microdomains of significantly lower lipid mobility are formed. The analysis of peptide FCS data confirms the presence of microdomains for anionic SLBs. No indications of microdomain formation are detected in SLBs composed purely of zwitterionic lipids. Additionally, our FCS results imply that arenicin-1 exists in the form of oligomers and/or aggregates when interacting with membranes of both compositions.
Citace poskytuje Crossref.org
Fluorescence Lifetime Correlation Spectroscopy (FLCS): concepts, applications and outlook
