Conjugation reactions in the preparations of quantum dot-based immunoluminescent probes for analysis of proteins by capillary electrophoresis
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Electrophoresis, Capillary MeSH
- Immunoassay instrumentation methods MeSH
- Quantum Dots * MeSH
- Luminescent Measurements instrumentation methods MeSH
- Molecular Probes chemistry MeSH
- Proteins analysis isolation & purification MeSH
- Antibodies chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Molecular Probes MeSH
- Proteins MeSH
- Antibodies MeSH
A number of biologically important molecules, such as DNA, proteins, and antibodies, are routinely conjugated with fluorescent tags for high-sensitivity analyses. Here, the application of quantum dots in the place of bright and size-tunable luminophores is studied. Several selected bioconjugation reactions via zero-length cross-linkers, long-chain linkers, and oriented methods for linking of quantum dots with proteins were tested. Anti-ovalbumin, anti-proliferating cell nuclear antigen, anti-hemagglutinin, and anti-CD3 membrane protein as model antibodies and annexin V were used as high-specificity selectors. The reaction yield and efficiency of the prepared immunoluminescent probes were tested by capillary zone electrophoresis with laser-induced fluorescence detection.
References provided by Crossref.org
Time-Dependent Growth of Silica Shells on CdTe Quantum Dots
