Nitrate reductase whole-cell assay: side effects associated with the use of benzyl viologen
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- analýza jednotlivých buněk MeSH
- bakteriální proteiny chemie genetika metabolismus MeSH
- benzylviologen metabolismus MeSH
- dusičnany metabolismus MeSH
- kinetika MeSH
- nitrátreduktasa chemie genetika metabolismus MeSH
- oxidace-redukce MeSH
- Rhodobacteraceae chemie enzymologie genetika MeSH
- transport elektronů MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- benzylviologen MeSH
- dusičnany MeSH
- nitrátreduktasa MeSH
A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV·](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV·](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV·](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.
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Arch Microbiol. 1996 Dec;166(6):361-7 PubMed
J Biol Chem. 1980 Jan 25;255(2):704-7 PubMed
J Bacteriol. 1991 Oct;173(19):5935-43 PubMed
Biochim Biophys Acta. 2005 Sep 5;1709(2):113-8 PubMed
J Biol Chem. 1984 Jun 10;259(11):7045-55 PubMed
Arch Biochem Biophys. 2003 Jan 15;409(2):327-34 PubMed
Biochem J. 1975 Jan;146(1):191-204 PubMed
J Bacteriol. 1971 Mar;105(3):1006-14 PubMed
FEBS Lett. 1990 Jun 4;265(1-2):85-7 PubMed
Biochem Biophys Res Commun. 1982 Oct 15;108(3):1126-30 PubMed
Trends Microbiol. 2005 Apr;13(4):175-80 PubMed
Biochem J. 1999 Nov 15;344 Pt 1:77-84 PubMed
Plasmid. 1995 Jul;34(1):11-21 PubMed
Appl Environ Microbiol. 1995 Aug;61(8):2852-8 PubMed
J Bacteriol. 1996 Apr;178(8):2328-33 PubMed
Eur J Biochem. 1974 Jun 15;45(2):343-9 PubMed
J Bacteriol. 1999 Nov;181(21):6706-11 PubMed
Arch Microbiol. 1997 Jan 29;167(1):61-6 PubMed
Mol Microbiol. 2002 Apr;44(1):143-55 PubMed
J Bacteriol. 1992 Nov;174(22):7316-20 PubMed
Mol Microbiol. 2008 Nov;70(3):667-81 PubMed
J Gen Microbiol. 1977 Jan;98(1):231-8 PubMed
Biochem J. 1977 Apr 15;164(1):199-211 PubMed
