A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.

Graduate Institute of Microbiology and Public Health National Chung Hsing University Taichung 402 Taiwan

Zobrazit více v PubMed

J Mol Biol. 1984 Nov 25;180(1):61-72 PubMed

Res Microbiol. 2009 Mar;160(2):152-8 PubMed

Folia Microbiol (Praha). 2010 May;55(3):286-94 PubMed

FEMS Microbiol Lett. 1999 Dec 15;181(2):217-23 PubMed

Lett Appl Microbiol. 2002;35(5):375-9 PubMed

Gene. 1990 Mar 1;87(1):123-6 PubMed

Gene. 1999 Feb 18;227(2):197-203 PubMed

Gene. 1995 Dec 1;166(1):175-6 PubMed

Gene. 1995 May 26;158(1):15-22 PubMed

Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PubMed

Antimicrob Agents Chemother. 2008 Mar;52(3):1198-200 PubMed

Nucleic Acids Res. 1997 Mar 15;25(6):1203-10 PubMed

J Bacteriol. 1995 Jul;177(14):4121-30 PubMed

Lett Appl Microbiol. 2008 Nov;47(5):393-8 PubMed