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Isolation, characterization and efficacy of phage MJ2 against biofilm forming multi-drug resistant Enterobacter cloacae
M. Jamal, S. Andleeb, F. Jalil, M. Imran, MA. Nawaz, T. Hussain, M. Ali, S. Ur Rahman, CR. Das,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
HEC-IRSIP- 2013
Higher Education Commision, Pakistan
- MeSH
- biofilmy růst a vývoj MeSH
- chlorid hořečnatý farmakologie MeSH
- chlorid vápenatý farmakologie MeSH
- délka genomu MeSH
- DNA virů MeSH
- Enterobacter cloacae virologie MeSH
- genom virový genetika MeSH
- hostitelská specificita MeSH
- koncentrace vodíkových iontů MeSH
- mikrobiální viabilita MeSH
- mnohočetná bakteriální léková rezistence * MeSH
- molekulová hmotnost MeSH
- odpadní voda virologie MeSH
- Podoviridae izolace a purifikace fyziologie ultrastruktura MeSH
- přichycení viru účinky léků MeSH
- teplota MeSH
- virové proteiny chemie MeSH
- Publikační typ
- časopisecké články MeSH
Biofilm is involved in a variety of infections, playing a critical role in the chronicity of infections. Enterobacter cloacae is a biofilm-forming and multi-drug-resistant (MDR) nosocomial pathogen leading to significant morbidity and mortality. This study aimed at isolation of a bacteriophage against MDR clinical strain of E. cloacae and its efficacy against bacterial planktonic cells and biofilm. A bacteriophage MJ2 was successfully isolated from wastewater and was characterized. The phage exhibited a wide range of thermal and pH stability and demonstrated considerable adsorption to host bacteria in the presence of CaCl2 or MgCl2. Transmission electron microscopy (TEM) showed MJ2 head as approximately 62 and 54 nm width and length, respectively. It had a short non-contractile tail and was characterized as a member of the family Podoviridae [order Caudovirales]. The phage MJ2 was found to possess 11 structural proteins (12-150 kDa) and a double-stranded DNA genome with an approximate size of 40 kb. The log-phase growth of E. cloacae both in biofilm and suspension was significantly reduced by the phage. The E. cloacae biofilm was formed under different conditions to evaluate the efficacy of MJ2 phage. Variable reduction pattern of E. cloacae biofilm was observed while treating it for 4 h with MJ2, i.e., biofilm under static conditions. The renewed media with intervals of 24, 72, and 120 h showed biomass decline of 2.8-, 3-, and 3.5-log, respectively. Whereas, the bacterial biofilm formed with dynamic conditions with refreshing media after 24, 72, and 120 h demonstrated decline in growth at 2.5-, 2.6-, and 3.3-log, respectively. It was, therefore, concluded that phage MJ2 possessed considerable inhibitory effects on MDR E. cloacae both in planktonic and biofilm forms.
Citace poskytuje Crossref.org
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- $a Jamal, Muhsin $u Department of Microbiology, Abdul Wali Khan University, Garden Campus, Mardan, 23200, Pakistan. muhsinjamal@awkum.edu.pk. Emerging Pathogens Institute (EPI), University of Florida (UF), Gainesville, FL, USA. muhsinjamal@awkum.edu.pk. College of Veterinary Sciences & Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan. muhsinjamal@awkum.edu.pk.
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- $a Isolation, characterization and efficacy of phage MJ2 against biofilm forming multi-drug resistant Enterobacter cloacae / $c M. Jamal, S. Andleeb, F. Jalil, M. Imran, MA. Nawaz, T. Hussain, M. Ali, S. Ur Rahman, CR. Das,
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- $a Biofilm is involved in a variety of infections, playing a critical role in the chronicity of infections. Enterobacter cloacae is a biofilm-forming and multi-drug-resistant (MDR) nosocomial pathogen leading to significant morbidity and mortality. This study aimed at isolation of a bacteriophage against MDR clinical strain of E. cloacae and its efficacy against bacterial planktonic cells and biofilm. A bacteriophage MJ2 was successfully isolated from wastewater and was characterized. The phage exhibited a wide range of thermal and pH stability and demonstrated considerable adsorption to host bacteria in the presence of CaCl2 or MgCl2. Transmission electron microscopy (TEM) showed MJ2 head as approximately 62 and 54 nm width and length, respectively. It had a short non-contractile tail and was characterized as a member of the family Podoviridae [order Caudovirales]. The phage MJ2 was found to possess 11 structural proteins (12-150 kDa) and a double-stranded DNA genome with an approximate size of 40 kb. The log-phase growth of E. cloacae both in biofilm and suspension was significantly reduced by the phage. The E. cloacae biofilm was formed under different conditions to evaluate the efficacy of MJ2 phage. Variable reduction pattern of E. cloacae biofilm was observed while treating it for 4 h with MJ2, i.e., biofilm under static conditions. The renewed media with intervals of 24, 72, and 120 h showed biomass decline of 2.8-, 3-, and 3.5-log, respectively. Whereas, the bacterial biofilm formed with dynamic conditions with refreshing media after 24, 72, and 120 h demonstrated decline in growth at 2.5-, 2.6-, and 3.3-log, respectively. It was, therefore, concluded that phage MJ2 possessed considerable inhibitory effects on MDR E. cloacae both in planktonic and biofilm forms.
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