Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21563303
DOI
10.1002/jbm.b.31832
Knihovny.cz E-resources
- MeSH
- Acrylamides chemistry MeSH
- Cell Line MeSH
- Embryonic Stem Cells cytology MeSH
- Hydrogels chemistry MeSH
- Humans MeSH
- Mice MeSH
- Oligopeptides chemistry MeSH
- Polymers chemistry MeSH
- Cell Proliferation MeSH
- Tissue Engineering methods MeSH
- Tissue Scaffolds * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acrylamides MeSH
- Hydrogels MeSH
- Oligopeptides MeSH
- poly(N,N-diethylacrylamide) MeSH Browser
- Polymers MeSH
Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,N-diethylacrylamide (DEAAm), N,N'-methylenebisacrylamide and methacrylic acid in the presence of (NH₄)₂SO₄ or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH₂, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH₂ pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSR-NH₂-modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.
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