Nine polymorphic microsatellite loci were developed for the spined loach, Cobitis taenia (Teleostei: Cobitidae). The loci were validated using 50 individuals from a population in Belgium. Moderate to high levels of polymorphism were detected (two to 11 alleles). In addition, most markers amplified successfully in three closely related taxa that are known to hybridize with C. taenia: C. elongatoides, C. taurica and C. tanaitica. Some of the loci are most likely diagnostic among species. These markers will be valuable for the study of the historical and contemporary interactions within C. taenia and the Cobitis species complex.

Research Institute for Nature and Forest Dwersbos 28 B 1630 Linkebeek Belgium Laboratory of Animal Diversity and Systematics K U Leuven Ch Deberiotstraat 32 B 3000 Leuven Belgium Laboratory of Fish Genetics Institute of Animal Physiology and Genetics Academy of Sciences of the Czech Republic Rumburská 89 277 21 Libéchov Czech Republic

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