Nucleobase modification as redox DNA labelling for electrochemical detection
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
21625726
DOI
10.1039/c1cs15049a
Knihovny.cz E-resources
- MeSH
- Staining and Labeling MeSH
- DNA analysis chemistry genetics MeSH
- Electrochemistry methods MeSH
- Humans MeSH
- Oligodeoxyribonucleotides chemistry MeSH
- Oxidation-Reduction MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- DNA MeSH
- Oligodeoxyribonucleotides MeSH
Basic aspects of DNA electrochemistry with a strong focus on the use of modified nucleobases as redox probes for electrochemical bioanalysis are reviewed. Intrinsic electrochemical properties of nucleobases in combination with artificial redox-active nucleobase modifications are frequently applied in this field. Synthetic approaches (both chemical and enzymatic) to base-modified nucleic acids are briefly summarized and their applications in redox labelling are discussed. Finally, analytical applications including DNA hybridization, primer extension, PCR, SNP typing, DNA damage and DNA-protein interaction analysis are presented (critical review, 91 references).
References provided by Crossref.org
