Isolation and phylogenetic classification of culturable psychrophilic prokaryotes from the Collins glacier in the Antarctica
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Bacteroides genetika izolace a purifikace MeSH
- biologické přípravky izolace a purifikace MeSH
- DNA bakterií analýza genetika MeSH
- fylogeneze * MeSH
- geologické sedimenty mikrobiologie MeSH
- ledový příkrov mikrobiologie MeSH
- molekulární sekvence - údaje MeSH
- Proteobacteria genetika izolace a purifikace MeSH
- ribozomální DNA analýza genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Antarktida MeSH
- Názvy látek
- biologické přípravky MeSH
- DNA bakterií MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
Culturable psychrophilic prokaryotes were obtained of samples of glacier sediment, seaside mud, glacier melted ice, and Deschampsia antarctica rhizosphere from Collins glacier, Antarctica. The taxonomic classification was done by a culture-dependent molecular approach involving the Amplified Ribosomal DNA Restriction Analysis. Two hundred sixty colonies were successfully isolated and sub-cultivated under laboratory conditions. The analysis showed a bacterial profile dominated by Beta-proteobacteria (35.2%) followed by Gamma-proteobacteria (18.5%), Alpha-proteobacteria (16.6%), Gram-positive with high GC content (13%), Cytophaga-Flavobacterium-Bacteroides (13%) and Gram-positive with low GC content (3.7%). Eleven of the isolates have been reported previously and the others microorganisms remain uncharacterized. The isolated microorganisms here could be a potential source for biotechnological products, such as cold-active enzymes and secondary metabolites.
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