The abc1-/coq8- respiratory-deficient mutant of Schizosaccharomyces pombe suffers from glutathione underproduction and hyperaccumulates Cd2+
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- ABC transportéry genetika MeSH
- delece genu * MeSH
- fenotyp MeSH
- glutathion metabolismus MeSH
- kadmium metabolismus MeSH
- mikrobiální testy citlivosti MeSH
- oxidace-redukce MeSH
- oxidancia farmakologie MeSH
- peroxidy metabolismus MeSH
- Schizosaccharomyces pombe - proteiny genetika MeSH
- Schizosaccharomyces účinky léků genetika metabolismus MeSH
- ubichinon genetika MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ABC transportéry MeSH
- ABC1 protein, S pombe MeSH Prohlížeč
- glutathion MeSH
- kadmium MeSH
- oxidancia MeSH
- peroxidy MeSH
- Schizosaccharomyces pombe - proteiny MeSH
- ubichinon MeSH
- ubiquinone 8 MeSH Prohlížeč
The abc1(-)/coq8(-) gene deletion respiratory-deficient mutant NBp17 of fission yeast Schizosaccharomyces pombe displayed a phenotypic fermentation pattern with enhanced production of glycerol and acetate, and also possessed oxidative stress-sensitive phenotypes to H(2)O(2), menadione, tBuOOH, Cd(2+), and chromate in comparison with its parental respiratory-competent strain HNT. As a consequence of internal stress-inducing mutation, adaptation processes to restore the redox homeostasis of mutant NBp17 cells were detected in minimal glucose medium. Mutant NBp17 produced significantly increased amounts of O(2)•- and H(2)O(2) as a result of the decreased internal glutathione concentration and the only slightly increased glutathione reductase activity. The Cr(VI) reduction capacity and hence the •OH production ability were decreased. The mutant cells demonstrated increased specific activities of superoxide dismutases and glutathione reductase (but not catalase) to detoxify at least partially the overproduction of reactive oxygen species. All these features may be explained by the decreased redox capacity of the mutant cells. Most notably, mutant NBp17 hyperaccumulated yellow CdS.
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