Changes in Caenorhabditis elegans life span and selective innate immune genes during Staphylococcus aureus infection
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Drug Resistance, Bacterial MeSH
- Caenorhabditis elegans genetics immunology metabolism MeSH
- Cysteine Proteases genetics immunology metabolism MeSH
- Gene Expression MeSH
- Host-Pathogen Interactions MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Lectins, C-Type genetics immunology metabolism MeSH
- Muramidase genetics immunology metabolism MeSH
- Colony Count, Microbial MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Immunity, Innate * MeSH
- Caenorhabditis elegans Proteins genetics immunology metabolism MeSH
- Staphylococcal Infections genetics immunology microbiology MeSH
- Staphylococcus aureus physiology MeSH
- Virulence MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cysteine Proteases MeSH
- Lectins, C-Type MeSH
- Muramidase MeSH
- Caenorhabditis elegans Proteins MeSH
Caenorhabditis elegans has been increasingly used to study the innate immunity and for the screening of microbe/host-specific pathogenic factors. Staphylococcus aureus-mediated infections with live C. elegans were performed on solid (full-lawn) and liquid assays. S. aureus required 90 ± 10 h for the complete killing of C. elegans, but the infection was started only after 32 h of exposure with 20% inoculum of S. aureus. The short time exposure studies revealed that, in 20% of inoculum, continuous exposure to the pathogen was required for the killing of nematode. In 100% of inoculum, only 8 h of exposure was sufficient to kill the C. elegans. To evaluate kinetically at the innate immune level, the regulation of representative candidate antimicrobial genes was investigated. Both semi-quantitative reverse transcriptase polymerase chain reaction (PCR) and real-time PCR analyses indicated the regulation of candidate immune regulatory genes of lysozyme (lys-7), cysteine protease (cpr-2), and C-type lectin (clec-60 and clec-87) family members during the course of S. aureus infections, indicating the possible contribution of the above players during the host immune response against S. aureus exposures.
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