Changes in Caenorhabditis elegans life span and selective innate immune genes during Staphylococcus aureus infection
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- bakteriální léková rezistence MeSH
- Caenorhabditis elegans genetika imunologie metabolismus MeSH
- cysteinové proteasy genetika imunologie metabolismus MeSH
- exprese genu MeSH
- interakce hostitele a patogenu MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lektiny typu C genetika imunologie metabolismus MeSH
- muramidasa genetika imunologie metabolismus MeSH
- počet mikrobiálních kolonií MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- přirozená imunita * MeSH
- proteiny Caenorhabditis elegans genetika imunologie metabolismus MeSH
- stafylokokové infekce genetika imunologie mikrobiologie MeSH
- Staphylococcus aureus fyziologie MeSH
- virulence MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cysteinové proteasy MeSH
- lektiny typu C MeSH
- muramidasa MeSH
- proteiny Caenorhabditis elegans MeSH
Caenorhabditis elegans has been increasingly used to study the innate immunity and for the screening of microbe/host-specific pathogenic factors. Staphylococcus aureus-mediated infections with live C. elegans were performed on solid (full-lawn) and liquid assays. S. aureus required 90 ± 10 h for the complete killing of C. elegans, but the infection was started only after 32 h of exposure with 20% inoculum of S. aureus. The short time exposure studies revealed that, in 20% of inoculum, continuous exposure to the pathogen was required for the killing of nematode. In 100% of inoculum, only 8 h of exposure was sufficient to kill the C. elegans. To evaluate kinetically at the innate immune level, the regulation of representative candidate antimicrobial genes was investigated. Both semi-quantitative reverse transcriptase polymerase chain reaction (PCR) and real-time PCR analyses indicated the regulation of candidate immune regulatory genes of lysozyme (lys-7), cysteine protease (cpr-2), and C-type lectin (clec-60 and clec-87) family members during the course of S. aureus infections, indicating the possible contribution of the above players during the host immune response against S. aureus exposures.
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Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10892-7 PubMed
Curr Biol. 2000 Dec 14-28;10(24):1615-8 PubMed
Mol Cell Biol. 2007 Aug;27(15):5544-53 PubMed
Infect Immun. 2003 Apr;71(4):2208-17 PubMed
Prog Nucleic Acid Res Mol Biol. 1993;45:207-32 PubMed
Proc Natl Acad Sci U S A. 1999 May 25;96(11):6048-53 PubMed
Proc Natl Acad Sci U S A. 2006 Sep 19;103(38):14086-91 PubMed
J Med Microbiol. 1995 Feb;42(2):91-5 PubMed
PLoS Pathog. 2010 Jul 01;6:e1000982 PubMed
Scand J Infect Dis. 2011 Apr;43(4):286-95 PubMed
Curr Biol. 2002 Jul 23;12(14):1209-14 PubMed
Microbiology (Reading). 2002 Oct;148(Pt 10):3235-3243 PubMed
Genome Biol. 2007;8(9):R194 PubMed
Genome Res. 2006 Aug;16(8):1005-16 PubMed
J Mol Evol. 2002 May;54(5):652-64 PubMed
Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):203-13 PubMed
PLoS Genet. 2006 Nov 10;2(11):e183 PubMed
