Phylogenetic analysis of urease-positive thermophilic Campylobacter (UPTC) strains based on the molecular characterization of the flaA gene
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Campylobacter lari classification genetics isolation & purification metabolism MeSH
- Campylobacter classification genetics isolation & purification metabolism MeSH
- Flagellin chemistry genetics metabolism MeSH
- Phylogeny MeSH
- Cloning, Molecular MeSH
- Bivalvia microbiology MeSH
- Molecular Sequence Data MeSH
- Seawater microbiology MeSH
- Open Reading Frames MeSH
- Polymerase Chain Reaction MeSH
- Recombinant Proteins chemistry genetics metabolism MeSH
- Amino Acid Sequence MeSH
- Sequence Analysis, DNA methods MeSH
- Sequence Alignment MeSH
- Urease metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- England MeSH
- Northern Ireland MeSH
- Names of Substances
- flaA protein, bacteria MeSH Browser
- Flagellin MeSH
- Recombinant Proteins MeSH
- Urease MeSH
Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.
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