Immunoproteomic assay of secreted proteins of Streptococcus suis serotype 9 with convalescent sera from pigs
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- 2D gelová elektroforéza MeSH
- antigeny bakteriální chemie imunologie MeSH
- bakteriální proteiny chemie imunologie metabolismus MeSH
- nemoci prasat imunologie mikrobiologie prevence a kontrola MeSH
- prasata MeSH
- proteomika metody MeSH
- rekonvalescence MeSH
- sérum chemie imunologie MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- Streptococcus suis genetika imunologie metabolismus MeSH
- streptokokové infekce imunologie mikrobiologie prevence a kontrola veterinární MeSH
- streptokokové vakcíny biosyntéza terapeutické užití MeSH
- vakcinace * MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Čína MeSH
- Názvy látek
- antigeny bakteriální MeSH
- bakteriální proteiny MeSH
- streptokokové vakcíny MeSH
Streptococcus suis is an important pathogen of pigs. In China, in addition to S. suis serotype 2, S. suis serotype 9 (SS9) is also a prevalent serotype. There is no vaccine available for SS9. An immunoproteome-based approach was developed to identify SS9 immunogenic proteins for vaccine development. Secreted proteins extracted from SS9 strain GZ0565 were screened by two-dimensional Western blotting using convalescent sera from pigs. Protein spots were excised from preparative gels and were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which led to the identification of ten immunogenic proteins (sortases, ABC transporter substrate-binding protein-maltose/maltodextrin, ABC transporter periplasmic protein, CHAP domain containing protein, peptidoglycan-binding LysM, elongation factor Tu, elongation factor G, thymidine kinase, molecular chaperone DnaK, hypothetical protein SSU98_2184). These novel immunogenic proteins, which are encoded by genes that are reasonably conserved among SS9 strains, may be developed as antigens for further study of SS9 vaccine.
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