Immunoproteomic assay of secreted proteins of Streptococcus suis serotype 9 with convalescent sera from pigs
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Antigens, Bacterial chemistry immunology MeSH
- Bacterial Proteins chemistry immunology metabolism MeSH
- Swine Diseases immunology microbiology prevention & control MeSH
- Swine MeSH
- Proteomics methods MeSH
- Convalescence MeSH
- Serum chemistry immunology MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Streptococcus suis genetics immunology metabolism MeSH
- Streptococcal Infections immunology microbiology prevention & control veterinary MeSH
- Streptococcal Vaccines biosynthesis therapeutic use MeSH
- Vaccination * MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- China MeSH
- Names of Substances
- Antigens, Bacterial MeSH
- Bacterial Proteins MeSH
- Streptococcal Vaccines MeSH
Streptococcus suis is an important pathogen of pigs. In China, in addition to S. suis serotype 2, S. suis serotype 9 (SS9) is also a prevalent serotype. There is no vaccine available for SS9. An immunoproteome-based approach was developed to identify SS9 immunogenic proteins for vaccine development. Secreted proteins extracted from SS9 strain GZ0565 were screened by two-dimensional Western blotting using convalescent sera from pigs. Protein spots were excised from preparative gels and were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which led to the identification of ten immunogenic proteins (sortases, ABC transporter substrate-binding protein-maltose/maltodextrin, ABC transporter periplasmic protein, CHAP domain containing protein, peptidoglycan-binding LysM, elongation factor Tu, elongation factor G, thymidine kinase, molecular chaperone DnaK, hypothetical protein SSU98_2184). These novel immunogenic proteins, which are encoded by genes that are reasonably conserved among SS9 strains, may be developed as antigens for further study of SS9 vaccine.
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Folia Microbiol (Praha). 2009;54(2):161-6 PubMed
Proteomics. 2008 Sep;8(17):3506-15 PubMed
Vaccine. 1997 Jun;15(8):834-8 PubMed
J Bacteriol. 2008 Nov;190(21):7079-86 PubMed
FEMS Microbiol Lett. 2006 Mar;256(1):1-15 PubMed
Proteomics. 2005 Dec;5(18):4777-93 PubMed
Appl Environ Microbiol. 2006 Sep;72(9):6355-63 PubMed
FEMS Immunol Med Microbiol. 2009 Jan;55(1):100-6 PubMed
J Biol Chem. 2009 Mar 13;284(11):6924-33 PubMed
Proteomics. 2001 Apr;1(4):480-93 PubMed
Microb Pathog. 2008 Sep;45(3):159-66 PubMed
Annu Rev Biochem. 2004;73:241-68 PubMed
Infect Immun. 2009 Jul;77(7):2957-61 PubMed
FEMS Immunol Med Microbiol. 1999 Aug 15;25(3):245-54 PubMed
Clin Microbiol Rev. 1999 Jan;12(1):19-39 PubMed
Vet Microbiol. 2000 Oct 1;76(3):259-72 PubMed
Microb Pathog. 1997 Jul;23(1):11-21 PubMed
FEMS Immunol Med Microbiol. 2008 Jun;53(1):52-9 PubMed
Microbes Infect. 2006 Jan;8(1):145-53 PubMed
Trends Biochem Sci. 2003 May;28(5):230-4 PubMed
Mol Microbiol. 2002 Feb;43(4):869-81 PubMed
Infect Immun. 2005 Jun;73(6):3342-50 PubMed
Mol Microbiol. 2009 May;72(3):779-94 PubMed
FEMS Microbiol Lett. 1996 Jan 1;135(1):103-10 PubMed
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