Matrix metalloproteinase 8 (MMP8) gene polymorphisms in chronic periodontitis
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21920499
DOI
10.1016/j.archoralbio.2011.08.018
PII: S0003-9969(11)00272-X
Knihovny.cz E-resources
- MeSH
- Bacterial Load MeSH
- Chronic Periodontitis genetics MeSH
- Adult MeSH
- Gene Frequency MeSH
- Genetic Predisposition to Disease MeSH
- Gingiva microbiology MeSH
- Haplotypes * MeSH
- Polymorphism, Single Nucleotide MeSH
- Middle Aged MeSH
- Humans MeSH
- Matrix Metalloproteinase 8 genetics MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Genetic * MeSH
- Sequence Analysis, DNA MeSH
- Case-Control Studies MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Matrix Metalloproteinase 8 MeSH
OBJECTIVE: Previous studies have suggested that some functional polymorphisms in the matrix metalloproteinase (MMPs) genes are associated with the risk of periodontal disease. However, to date no study has investigated MMP8 gene variants in relation to chronic periodontitis (CP). The aim of this study was to analyse polymorphisms in the MMP8 gene and their associations with microbial composition and clinical manifestation of CP. DESIGN: A total of 619 unrelated Czech subjects were included in the present study. Two polymorphisms [-799C/T (rs11225395) and +17C/G (rs2155052)] in the MMP8 gene were studied in 341 patients with CP and 278 unrelated non-periodontitis controls. Both polymorphisms were detected using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. Subgingival bacterial colonisation (occurrence of bacteria in subgingival pockets and gingival sulci) was investigated by a commercial semiquantitative kit in selected subjects (N=169). RESULTS: Our results showed no differences in the allele and genotype frequencies of the MMP8 -799C/T and +17C/G polymorphisms between patients with CP and controls (p>0.05). Nevertheless, the haplotype T(-799)/C(+17) was significantly more frequent in patients with CP than in controls [43.7% vs. 37.6%, p<0.05, OR=1.273 (95% CI: 1.013-1.601)]. Despite significant differences determined in the occurrence of periodontal bacteria between patients with CP and non-periodontitis controls (from p<0.000001 to p<0.05), no significant relationships between periodontal pathogens, MMP8 polymorphisms and CP were found (p>0.05). CONCLUSIONS: Although none of the investigated SNPs in the MMP8 gene was individually associated with periodontitis, specific haplotype showed association with clinical manifestation of chronic periodontitis in a Czech population.
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