Sample processing and methodological pitfalls in multiple myeloma research
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
21923059
Knihovny.cz E-resources
- MeSH
- Biological Specimen Banks MeSH
- Biomedical Research MeSH
- Bone Marrow pathology MeSH
- Humans MeSH
- Multiple Myeloma diagnosis MeSH
- Specimen Handling MeSH
- Plasma Cells classification immunology MeSH
- Flow Cytometry methods MeSH
- Syndecan-1 analysis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Syndecan-1 MeSH
In this paper, initial processing of biological material, cell separation algorithms and other procedures are discussed. For samples with initial infiltration of plasma cells > 5%, CD138 MicroBeads and Auto-Magnetic-Activated Cell Sorting program are used. Fluorescence-Activated Cell Sorting is used exclusively for cell populations with low-abundance; these samples are detected using fluorescently labeled antibodies only. Isolated plasma cells are further processed for molecular biological studies, for cytogenetics and protein analyses. Furthermore, this work examines the pitfalls of research related to multiple myeloma; some of them we have overcome, while the others are still problematic.
Global DNA methylation and increased DNMT3A expression in multiple myeloma patients
