Tissue repair driven by two different mechanisms of growth factor plasmids VEGF and NGF in mice auricular cartilage: regeneration mediated by administering growth factor plasmids
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- chondrogeneze * účinky léků fyziologie MeSH
- fyziologická neovaskularizace * účinky léků fyziologie MeSH
- genetické vektory MeSH
- hojení ran * účinky léků fyziologie MeSH
- krysa rodu Rattus MeSH
- kůže zranění MeSH
- látky indukující angiogenezi aplikace a dávkování MeSH
- modely u zvířat MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- neurotrofní faktory * aplikace a dávkování genetika MeSH
- plazmidy MeSH
- ušní chrupavka zranění fyziologie MeSH
- vaskulární endoteliální růstový faktor A * aplikace a dávkování genetika MeSH
- zánět metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- látky indukující angiogenezi MeSH
- neurotrofní faktory * MeSH
- vaskulární endoteliální růstový faktor A * MeSH
The focus of this study was to compare the role of nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) in the regeneration of experimental skin and cartilage trauma. The role of VEGF in this process is known since decade; the NGF participation on this process has been first discussed within the spinal cord injury repair. We hypothesized that both VEGF and NGF induce angiogenesis and take part on the repair process. The angiogenesis response and the cartilage regeneration after phVEGF(165) plasmid and rat pcNGF plasmid administration were investigated using BALB/c mice. PhVEGF(165) and pcNFG were injected into the right mice ear and plain vector injection into the left ear the day before trauma. The next day, all mice were ear-punched, resulting in 2-mm diameter puncture through the center of both pinnae. In BALB/c mouse strain, a significantly faster cartilage repair was observed after phVEGF(165) and pcNGF injection into punched ear area in comparison to the control group. It has been shown that the healing process is after VEGF and NGF injection driven differentially. In case of VEGF is the cartilage wound repaired by induction of new chondrocytes differentiation. In the case of NGF, the regeneration is supported by immature leukocytes attracted into the punched area. The leukocytes induct angiogenesis so far indirectly by inflammation. The NGF-induced inflammation environment may be a part of mosaic creating the complete picture of regeneration.
Zobrazit více v PubMed
Science. 1992 Sep 4;257(5075):1401-3 PubMed
Arch Dermatol Res. 2001 Jun;293(6):291-5 PubMed
Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9656-61 PubMed
Ann Thorac Surg. 1991 Aug;52(2):258-64 PubMed
Circulation. 1993 Oct;88(4 Pt 1):1534-57 PubMed
Circulation. 2002 Oct 22;106(17):2257-62 PubMed
Biochem Biophys Res Commun. 2003 Oct 10;310(1):143-7 PubMed
Curr Top Microbiol Immunol. 1999;237:97-132 PubMed
J Oral Maxillofac Surg. 1988 May;46(5):391-8 PubMed
J Mol Cell Cardiol. 1997 May;29(5):1321-30 PubMed
Nature. 2000 Sep 14;407(6801):242-8 PubMed
Lancet. 1996 Aug 10;348(9024):370-4 PubMed
Biochem Biophys Res Commun. 2007 May 18;356(4):919-24 PubMed
Diabetologia. 2004 Jun;47(6):1047-54 PubMed
Nat Med. 1999 Jun;5(6):623-8 PubMed
Plast Reconstr Surg. 1989 Jun;83(6):1013-9; discussion 1020-1 PubMed
Basic Res Cardiol. 2005 Mar;100(2):121-30 PubMed
Rheumatology (Oxford). 2002 Dec;41(12):1413-8 PubMed
J Exp Med. 1998 Feb 2;187(3):297-306 PubMed
FASEB J. 2002 Aug;16(10):1307-9 PubMed
J Clin Invest. 1994 Nov;94(5):1757-63 PubMed
J Cell Sci. 2000 Jan;113 ( Pt 1):59-69 PubMed
Science. 1987 Sep 4;237(4819):1154-62 PubMed
Circulation. 1996 Dec 15;94(12):3281-90 PubMed
Ann Anat. 2005 Nov;187(5-6):509-19 PubMed
Circulation. 1998 Sep 29;98(13):1261-3 PubMed
J Immunol. 1993 May 15;150(10):4478-85 PubMed
Ann N Y Acad Sci. 1987;496:182-91 PubMed
Arthritis Res. 2002;4 Suppl 3:S99-107 PubMed
